Effect of Different Cryopreservation Regimens on Some Characteristics of Embryonic Neuronal Cells

Authors

  • A. N. Goltsev
  • T. M. Gurina
  • N. N. Babenko
  • M. V. Ostankov

Keywords:

cryopreservation, embryonic neuronal cells, overcooling

Abstract

The effect of different cryopreservation regimens on the integrity of embryonic neuronal cells (ENC) according to the criteria of nuclear cell number in suspension, their viability and adhesive potential was studied. There was noted the dependency of the ENC morphofunctional characteristics both on cooling rate and the overcooling value of a sample. The possibility of a selective effect of different cryopreservation regimens on a subpopulation composition of embryonic brain cells was under consideration.

Author Biographies

A. N. Goltsev

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

T. M. Gurina

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

N. N. Babenko

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

M. V. Ostankov

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Published

2003-05-15

How to Cite

Goltsev, A. N., Gurina, T. M., Babenko, N. N., & Ostankov, M. V. (2003). Effect of Different Cryopreservation Regimens on Some Characteristics of Embryonic Neuronal Cells. Problems of Cryobiology and Cryomedicine, (1), 46-50. Retrieved from http://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/1009

Issue

Section

Cryopreservation of Biological Resources