Effect of Amphiphilic Compounds on the Susceptibility of Partially Dehydrated Erythrocytes to Hypertonic Stress

Authors

  • N. V. Orlova
  • N. M. Shpakova

Keywords:

erythrocytes, amphiphilic compounds, hypertonic stress, antihaemolytic activity

Abstract

We have studied the effect of various amphiphilic compounds on the susceptibility of partially dehydrated erythrocytes to a hypertonic stress (4.0M NaCl, 37oC). There has been found a high antihaemolytic activity of the representatives of cationic, anionic, nonionic, zwitterionic amphiphilic compounds when removing the cells out of 0.45 and 0.7 M NaCl into the medium, containing 4.0 M NaCl. The values of antihaemolytic activity depended both on the peculiarities of the compounds studied, and on the initial state of cells, subjected to a stress effect.

Author Biographies

N. V. Orlova

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

N. M. Shpakova

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Published

2003-05-15

How to Cite

Orlova, N. V., & Shpakova, N. M. (2003). Effect of Amphiphilic Compounds on the Susceptibility of Partially Dehydrated Erythrocytes to Hypertonic Stress. Problems of Cryobiology and Cryomedicine, (1), 59-64. Retrieved from http://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/1011

Issue

Section

Cryopreservation of Biological Resources