Study of Microorganisms’ Viability and Integrity of Structural Sites of Their DNA, Amplified in PCR After Low-Temperature Storage under Different Conditions

Authors

  • I. P. Vysekantsev
  • E. A. Omelchenko
  • T. M. Gurina

Keywords:

microorganisms, cryopreservation, DNA, PCR

Abstract

The obtained experimental data testify to the fact that under low temperature preservation the cyclic changes in storage temperature from –196 to –100°C can result in additional death of microbial cells. In microbial cells, stored under liquid nitrogen constant temperature and under conditions of storage temperature variation within the mentioned range, there are preserved the DNA structural sites, providing the amplification in polymerase chain reaction (PCR) of specific fragments.

Author Biographies

I. P. Vysekantsev

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

E. A. Omelchenko

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

T. M. Gurina

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Downloads

Published

2003-05-15

How to Cite

Vysekantsev, I. P., Omelchenko, E. A., & Gurina, T. M. (2003). Study of Microorganisms’ Viability and Integrity of Structural Sites of Their DNA, Amplified in PCR After Low-Temperature Storage under Different Conditions. Problems of Cryobiology and Cryomedicine, (1), 71–75. Retrieved from http://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/1013

Issue

No. 1 (2003): Problems of Cryobiology

Section

Cryopreservation of Biological Resources

License

Authors who publish with this journal agree to the following terms:

  • Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  • Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  • Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).