Modifying Effect of Amphipatic Compounds on Erythrocyte Sensitivity to the Change in the Medium Osmotic and Temperature Conditions

  • F. Abualasal
  • S. V. Melikhova
  • V. A. Bondarenko
Keywords: erythrocytes, amphipatic compounds, hypertonic stress, hypertonic cryohemolysis

Abstract

The effect of amphipatic compounds on the erythrocyte sensitivity to hypertonic stress when transferring into 4.0 M NaCl and hypertonic cryohemolysis under cooling from 37 down to 0°C was investigated. It was established, that independently on the nature of the acting agent its effect on cell preservation under hypertonic hemolysis and hypertonic stress was the similar. The concentration range, where the protective effect is maximum, is manifested under hypertonic stress for each studied compound. Under high amphipate concentrations under hypertonic conditions the prohemolytic effect of the compound is noted. When transferring into 4.0 M NaCl the protective effect of amphipate is also manifested in a change of temperature dependence of erythrocyte susceptibility to hypertonic stress.

Author Biographies

F. Abualasal

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

 

S. V. Melikhova

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

 

V. A. Bondarenko

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

 

Published
2003-09-25
How to Cite
Abualasal, F., Melikhova, S. V., & Bondarenko, V. A. (2003). Modifying Effect of Amphipatic Compounds on Erythrocyte Sensitivity to the Change in the Medium Osmotic and Temperature Conditions. Problems of Cryobiology and Cryomedicine, (3), 30-37. Retrieved from http://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/1056
Section
Theoretical and Experimental Cryobiology