Dynamics of Lactate Accumulation in Human Spermatozoa during Perftoran Introduction into Hypothermic Storage Medium

Authors

  • I. V. Cherkashina

Keywords:

spermatozoa, perftoran, hypotermy, lactate, motility

Abstract

There was studied the effect of a gas-transporting blood-substitute Perftoran on lactic acid content and spermatozoa motility under hypothermic storage conditions at 4°C. It has been demonstrated that perftoran (PF) adding into the incubation medium combined with the effect of methylxantine derivatives at the stage of complete stopping of cells gives the possibility to prolong the storage time of spermatozoa up to 120 hrs. The change of lactate content in incubation medium with PF testifies to the possibility of human spermatozoa to change for aerobic metabolism following 72 hrs’ hypothermic storage.

Author Biography

I. V. Cherkashina

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

 

Downloads

Published

2003-09-25

How to Cite

Cherkashina, I. V. (2003). Dynamics of Lactate Accumulation in Human Spermatozoa during Perftoran Introduction into Hypothermic Storage Medium. Problems of Cryobiology and Cryomedicine, (3), 75–79. Retrieved from http://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/1062

Issue

No. 3 (2003): Problems of Cryobiology

Section

Cryopreservation of Biological Resources

License

Authors who publish with this journal agree to the following terms:

  • Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  • Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  • Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).