Effect of PEG-1500 on Dipyridamole Binding with Erythrocyte Anion Channel

Authors

  • V. V. Ramazanov
  • Ya. O. Nardid
  • O. A. Olejnik
  • V. A. Bondarenko

Keywords:

erythrocytes, anion channel, posthypertonic lysis, cytoskeleton

Abstract

The authors studied the effect of PEG-1500 on ability of dipyridamole to inhibit the H+ ions transport in erythrocytes. It was established that the inhibitor was not bound in anion channel of erythrocytes ghosts in sulfate medium, despite its ability to block the H+ ions entrance into cells. Using of fluoride loaded erythrocytes allowed to assume that dipyridamole’s inhibitory ability in cells placed to sulfate medium is controlled by chloride output flow during Jacobs-Stewart cycle functioning, and that proton transport does not reflect the whole amount of entering sulfate. Dipyridamole blockage of stated fraction of H+ ions transport was considerably withdrawn in PEG-1500 presense due to inhibitor’s redistribution from membrane to cryoprotectant solution.

Author Biographies

V. V. Ramazanov

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

 

Ya. O. Nardid

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

 

O. A. Olejnik

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

 

V. A. Bondarenko

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

 

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Published

2003-12-25

How to Cite

Ramazanov, V. V., Nardid, Y. O., Olejnik, O. A., & Bondarenko, V. A. (2003). Effect of PEG-1500 on Dipyridamole Binding with Erythrocyte Anion Channel. Problems of Cryobiology and Cryomedicine, (4), 12–19. Retrieved from http://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/1073

Issue

No. 4 (2003): Problems of Cryobiology

Section

Theoretical and Experimental Cryobiology

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