Influence of Hypoxia and Hypercapnia on Fatty Acid Composition of Lipids in White Muscles of Common Carp Cyprinus carpio

Sergii V. Sysoliatin, Svitlana V. Midyk, Svitlana V. Khyzhnyak

Abstract


Study of the involvement of fish tissue fatty acids into the organism reactivity caused by an influence of exogenous factors is crucial for investigation of cell mechanisms underlying hypobiotic effect. Gas chromatography was used to estimate the composition and quantities of fatty acids of total lipids in white muscles of the Ukrainian scaly carp species. Twenty eight fatty acids were identified and their quantitative redistribution was revealed under hypoxic-hypercapnic action following a decrease in the environmental temperature (artificial hibernation). The decrease in total content of saturated and an increase in the one of unsaturated fatty acids was mainly due to the ω-3 and ω-6 polyunsaturated acids family. The optimum ω-3/ω-6 ratio under the studied conditions was supposedly maintained due to acyl-lipid ω-3 and ω-6-desaturase activity. Modified content of total lipids fatty acids in carp white muscles was likely a part of the cell mechanism of hypobiosis factors action in the fish organism.

Probl Cryobiol Cryomed 2017; 27(3): 195–202


Keywords


fatty acids; white muscles; carp; artificial hibernation; hypercapnia; hypoxia

Full Text:

PDF

References


Bell M.V., Dick J.R., Porter A.E. Biosynthesis and tissue deposition of docosahexaenoic aced (22:6n–3) in rainbow trout (Oncorhynchus mykiss). Lipids 2001; 36 (10): 1153–1159. CrossRef PubMed

Christie W.W. Lipid Analysis: Isolation, Separation, Identification and Structural Analysis of Lipids. 2nd edn – Oxford: Pergamon Press, 1982. – 207 p.

Fedorchenko C.V., Kurt S. Chromatographic analytic methods: Manual: Ivano-Frankivsk: 2012.

Folch J., Lees M., Sloane-Stanley G.H. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem 1957; (226): 497–509. PubMed

Guschina L.A., Harwood J. L. Mechanisms of temperature adaptation in poikilotherms. FEBS Lett 2006; 580 (23): 5477–5483. CrossRef PubMed

Gulevsky A.K., Shchenyavsky I.I., Relina L.I. et al. Cold adaptation of poikilothermal and heterothermal animals. In: Goltsev A.N., editor. Actual of Problems of Cryobiology. Kharkiv; 2012. P. 127–164.

Hong H., Zhou Y., Wu H. et al. Lipid content and fatty acid profile of muscle, brain and eyes of seven fresh water fish: a comparative study. J Am Oil Chem Soc 2014; 91(5): 795–804. CrossRef

Kogteva G.S., Bezuglov V.V. Unsaturated fatty acids as endogenous bioregulators. Review. Biokhimiya 1998; 63 (1): 6–15.

Khyzhnyak S.V., Voitsitsky V.M., Melnychuk S.D. Energy function of mitochondria at hipobiosis: Kiev, 2016.

Khyzhnyak S.V., Midik S.V., Sysolyatin S.V., Voitsitsky V.M. Content of fatty acids of lipids of different organs of rats after hypoxyhypercapnic influence. Biol Tvarin 2016; 18 (2): 125–132. CrossRef

Lovern J.A. The lipids of marine organisms. Oceanogr Mar Biol 1964; (2): 169–191.

Melnichuk S.D., Melnichuk D.O., Tereshchenko S.V. The method of transfer and storage of fish in a state of artificial hibernation and installation for its implementation. Patent of Ukraine №37303А, IPC А01К63/02. 2001 May 15.

Murzina S.A., Nefedova Z.A., Nemova N.N. Influence of fatty acids (markers of food sources of fish) on the mechanisms of adaptation in the conditions of high twins (review). Proceedings of the Karelian Research Center of Russian Academy of Sciences 2012; (2): 18–25.

Nazarov P.E., Groza N.V. Polyunsaturated fatty acids as universal biogenic endoregulators. Vestnik MITHT 2009; 4 (5): 3–19.

Popova E.M., Koschey I.V. Lipids as part of adaptation to environmental stress. Fisheries Science of Ukraine 2007; (1): 49–56.

Sidorov V.S. Ecological fish biochemistry. Lipids. Leningrad: Science; 1983.

Sysolyatin S.V. The lipid composition of tissue of scaly carp (Cyprinus Carpio L.) in the conditions of artificial carbon hibernation. Fisheries Science of Ukraine 2016; 3(37): 111–122.

Timofeev N.N. Hypobiosis and cryobiosis. Past, present, future. Moscow, 2005.

Tocher D.R. Metabolism and functions of lipids and fatty acids in teleost fish. Rev Fish Sci 2003; (11): 107–184. CrossRef

Velansky P.V., Kostetsky E.Ya. Lipids of sea cold-water fish. Biologia Morya 2008; 34(1): 53–57.




DOI: https://doi.org/10.15407%2Fcryo27.03.195

Refbacks

  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

 

Institute for Problems of Cryobiology and Cryomedicine

23, Pereyaslavskaya str., Kharkov, Ukraine

Tel. +38057 373 4143; Fax +38057 373 5952

e-mail: journal@cryo.org.ua