Experimental Substantiation of Therapeutic Hypothermia and Cell Therapy Application at Dyscirculatory Encephalopathy in SHR Rats. Part 1. Spontaneously Hypertensive SHR Rats as a Model of Dyscirculatory Encephalopathy

Victoria S. Aidarova, Vladislav G. Babiichuk, Olga V. Kudokotseva, O. V. Naumova, Ivan I. Lomakin, E. S. Protsenko


The possibility of using spontaneously hypertensive rats (SHR) as an adequate of dyscirculatory encephalopathy model was investigated. Morphological, morphometric parameters and intensity of processes of lipid peroxidation (malonic dialdehyde (MDA) content) in the brain tissue of white outbred rats (assumed as a normotensive control) and SHR rats were assessed, as well as their blood pressure, blood viscosity and hematocrit were analyzed. In the SHR rats, the changes in architectonics of the brain vascular bed and degenerative-dystrophic lesions of the brain tissue were noted on the background of significantly high blood pressure, increased blood viscosity, reduced oxygen delivery to tissues, and high MDA content (compared with normotensive control). It has been established that SHR rats can be used for developing the ways of correction of pathologically changed brain structures using the methods of craniocerebral hypothermia as well as introduction of cryopreserved cells of cord blood.


Probl Cryobiol Cryomed 2018; 28(3): 224236


dyscirculatory encephalopathy; arterial hypertension; brain; lipid peroxidation

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Amenta F, Di Tullio MA, Tomassoni D. Arterial hypertension and brain damage-evidence from animal models (review). Clin Exp Hypertens. 2003; 25(6): 359–80. CrossRef PubMed

Anishchenko AM, Aliev OI, Sidekhmenova AV, et al. Dynamics of blood pressure elevation and endothelial dysfunction in SHR rats during the development of arterial hypertension. Bull Exp Biol Med. 2015; 159(5): 591–3. CrossRef PubMed

Bakris GL, Sorrentino M. Hypertension: A companion to braunwald's heart disease. Philadelphia: Elsevier, 2018. 520 p.

Cheng J, Liu A, Shi MY, Yan Z. Disrupted glutamatergic transmission in prefrontal cortex contributes to behavioral abnormality in an animal model of ADHD. Neuropsychopharmacology. 2017; 42: 2096–104. CrossRef PubMed

Coca A. Hypertension and brain damage. NY: Springer International Publishing, 2016. 329 p.

De Deyn PP, Dam DV, editors. Animal models of dementia. NY: Humana Press, 2011. 729 p. CrossRef

Doris PA. Genetics of hypertension: an assessment of progress in the spontaneously hypertensive rat. Physiol Genomic. 2017; 49(11): 601–17. CrossRef PubMed

Fedorova TN, Korshunova TS, Larsky EG. [Reaction with TBA for determination of malonic dialdehyde of blood by fluorescence method]. Lab. Delo. 1983; (3): 25–7. Russian.

Golovchenko YuI, Treshchinskaya MA. [Pathogenetic features of the development of circulatory brain hypoxia in hypertension]. Meditsina Neotlozhnyh Sostoyaniy. 2011; 35(4): 86–93. Russian.

Plotnikov MB, Aliev OI, Anishchenko AM, et al. [Dynamics of blood pressure and quantity indices of erythrocytes in SHR in early period of arterial hypertension forming]. Ross Fiziol Zh Im I M Sechenova. 2015; (7): 822–8. Russian. PubMed

Plotnikov MB, Aliev OI, Nosarev AV, et al. Relationship between arterial blood pressure and blood viscosity in spontaneously hypertensive rats treated with pentoxifylline. Biorheology. 2016 Jul 26; 53(2): 93–107. CrossRef PubMed

Plotnikov MB, Aliev OI, Shamanaev AY, et al. Effects of pentoxifylline on hemodynamic, hemorheological, and microcirculatory parameters in young SHRs during arterial hypertension development. Clin Exp Hypertens. 2017; 39(6): 570–8. CrossRef PubMed

Plotnikov MB, Aliev OI, Sidekhmenova AV, et al. Dihydroquercetin improves microvascularization and microcirculation in the brain cortex of SHR rats during the development of arterial hypertension. Bull Exp Biol Med. 2017 May; 163(1): 57–60. CrossRef PubMed

Rostron CL, Gaeta V, Brace LR, Dommett EJ. Instrumental conditioning for food reinforcement in the spontaneously hypertensive rat model of attention deficit hyperactivity disorder. BMC Res Notes [Internet]. 2017 [cited 2018 Feb. 4]; 10(1): 525. Available from: https://bmcresnotes.biomedcentral.com/track/pdf/10.1186/s13104-017-2857-5. CrossRef

Sabbatini M, Catalani A, Consoli C, et al. The hippocampus in spontaneously hypertensive rats: an animal model of vascular dementia? Mech Ageing Dev. 2002; 123(5): 547–59. CrossRef

Sokolova IB, Polyntsev DG. [Efficacy of mesenchymal stem cells used for the improvement cerebral microcirculation in spontaneously hypertensive rats]. Tsitologiia. 2017; 59(4): 279–84. Russian.

Sokolova IB, Sergeev IV, Dvoretsky DP. Influence of high blood pressure on microcirculation in cerebral cortex of young rats. Bull Exp Biol Med. 2016 Jan;160(3):298–9. CrossRef PubMed

Sokolova IB, Sergeev IV, Fedotova OR, Dvoretsky DP. Age-related changes of microcirculation in pia mater of rats' sensorimotor cortex. Advances Gerontol. 2016; 29(4): 567–72. PubMed

Tayebati SK, Tomassoni D, Amenta F. Spontaneously hypertensive rat as a model of vascular brain disorder: microanatomy, neurochemistry and behavior. J Neurol Sci. 2012; 322(1–2): 241–9. CrossRef PubMed

Tomassoni D, Amenta F, Amantini C, et al. Brain activity of thioctic acid enantiomers: In vitro and in vivo studies in an animal model of cerebrovascular injury. Int J Mol Sci. 2013; 14(3): 4580–95. CrossRef PubMed

Yarygin VN, editors. [Regenerative biology and medicine. Book II. Cellular technologies in the treatment of diseases of the nervous system]. Omsk: Omsk Regional Printing House; 2015. 360 p. Russian.

Zhuravlev DA. Hypertension models. Spontaneously hypertensive rats. Arterial Hypertension. 2009; 15(6): 721–2.

DOI: https://doi.org/10.15407/cryo28.03.224


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