Experimental Substantiation of Therapeutic Hypothermia and Cell Therapy Application at Dyscirculatory Encephalopathy in SHR Rats. Part 1. Spontaneously Hypertensive SHR Rats as a Model of Dyscirculatory Encephalopathy

Authors

  • Victoria S. Aidarova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Vladislav G. Babiichuk Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Olga V. Kudokotseva Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • O. V. Naumova Kharkiv National Medical University, Kharkiv
  • Ivan I. Lomakin Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • E. S. Protsenko V.N. Karazin Kharkiv National University, Kharkiv

DOI:

https://doi.org/10.15407/cryo28.03.224

Keywords:

dyscirculatory encephalopathy, arterial hypertension, brain, lipid peroxidation

Abstract

The possibility of using spontaneously hypertensive rats (SHR) as an adequate of dyscirculatory encephalopathy model was investigated. Morphological, morphometric parameters and intensity of processes of lipid peroxidation (malonic dialdehyde (MDA) content) in the brain tissue of white outbred rats (assumed as a normotensive control) and SHR rats were assessed, as well as their blood pressure, blood viscosity and hematocrit were analyzed. In the SHR rats, the changes in architectonics of the brain vascular bed and degenerative-dystrophic lesions of the brain tissue were noted on the background of significantly high blood pressure, increased blood viscosity, reduced oxygen delivery to tissues, and high MDA content (compared with normotensive control). It has been established that SHR rats can be used for developing the ways of correction of pathologically changed brain structures using the methods of craniocerebral hypothermia as well as introduction of cryopreserved cells of cord blood.

 

Probl Cryobiol Cryomed 2018; 28(3): 224–236

Author Biographies

Victoria S. Aidarova, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryophysiology

Vladislav G. Babiichuk, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryophysiology

Olga V. Kudokotseva, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryophysiology

Ivan I. Lomakin, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryophysiology

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Published

2018-09-27

How to Cite

Aidarova, V. S., Babiichuk, V. G., Kudokotseva, O. V., Naumova, O. V., Lomakin, I. I., & Protsenko, E. S. (2018). Experimental Substantiation of Therapeutic Hypothermia and Cell Therapy Application at Dyscirculatory Encephalopathy in SHR Rats. Part 1. Spontaneously Hypertensive SHR Rats as a Model of Dyscirculatory Encephalopathy. Problems of Cryobiology and Cryomedicine, 28(3), 224–236. https://doi.org/10.15407/cryo28.03.224

Issue

Vol. 28 No. 3 (2018): Probl Cryobiol Cryomed

Section

Cryomedicine, Clinical and Experimental Transplantology

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