Infectious Activity of L. Pasteur Rabies Virus Vaccine Strain Frozen-Dried in Various Protective Media And Then Stored at Various Temperatures

Viktoriia V. Varianytsia, Igor P. Vysekantsev

Abstract


In this research, we studied the survival of industrial L. Pasteur rabies virus vaccine strain  after freeze-drying in various protective media and further storage at 5, –20 and –80°C. After 24-month storage (the observation period), the highest rates of the virus infectious activity were found after storage at –80°C in all the media. The maximum virus survival was found in the DMEM-based protective medium, supplemented with 0.5% bovine serum albumin, 1% gelatin and 5% sucrose. The infectious activity of the specimens, frozen-dried in the media, containing either 5% sucrose or 3% gelatin and 5% sucrose, or 10% sucrose was significantly lower. The supplement of 3% gelatin to a protective medium was established to significantly increase the time of preparations dissolution as compared to other media and the regulation standard (i. e., not more than 1 min), that could complicate their practical use. The time of specimens’ dissolution with 1% gelatin and 5% sucrose or 10% sucrose was significantly longer if comaring to those with 5% sucrose, but complied with the production regulations

 

Probl Cryobiol Cryomed 2018; 28(4): 333-342


Keywords


rabies virus; freeze-drying; cell culture; protective media; long-term storage; virus safety; infectious activity of virus

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DOI: https://doi.org/10.15407/cryo28.04.333

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