Glycerol Impact on Human and Bovine Erythrocytes Under Hypertonic Shock

Authors

  • Olena Ye. Nipot Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Natalya A. Yershova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Olga O. Shapkina Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Sergiy S. Yershov Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Vadim M. Kuchkov Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

DOI:

https://doi.org/10.15407/cryo29.03.199

Keywords:

mammalian erythrocytes, hypertonic shock, penetrating cryoprotectant, glycerol, incubation temperature, hemolysis

Abstract

Here, we have studied the impact of preliminary incubation of human and bovine erythrocytes in glycerol solutions on their sensitivity to hypertonic shock. The cell damage was shown to significantly reduce with increasing the glycerol concentration. The ranges of a sharp change in human and bovine cell sensitivity were from 0.1 to 0.5 and 0.5 to 1 M, respectively. A change in incubation time with glycerol from 1 to 10 min resulted in sensitivity decrease to hypertonic shock for both mammalian species, but the curves of dependence were different. A damaging effect of hypertonic shock increased with temperature rise from 0 up to 10°C for human erythrocytes and from 0 to 25°C for bovine ones. Preliminary erythrocyte incubation with glycerol entailed a decreased hypertonic damage of erythrocytes at all the studied temperatures for human cells and at those above 5°C for bovine cells. Our findings and the reported data review suggested about a different protective mechanism for human and bovine erythrocytes using glycerol under hypertonic shock conditions.

 

Probl Cryobiol Cryomed 2019; 29(3):199–205.

Author Biographies

Olena Ye. Nipot, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryocytology

Natalya A. Yershova, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryocytology

Olga O. Shapkina, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryocytology

Sergiy S. Yershov, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryocytology

Vadim M. Kuchkov, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryocytology

References

Aleksandrova DI, Orlova NV, Shpakova NM. Comparative study of preliminarily dehydrated human and bovine erythrocyte sensitivity to hypertonic stress. Problems of Cryobiology. 2007; 17(4): 327-34.

Benga G. Comparative studies of water permeability of red blood cells from humans and over 30 animal species: an overview of 20 years of collaboration with Philip Kuchel. Eur Biophys J. 2013; 42(1): 33-46. CrossRef

Campos E, Moura TF, Oliva A, et al. Lack of aquaporin 3 in bovine erythrocyte membranes correlates with low glycerol permeation. Biochem Biophys Res Comm. 2011; 408(3): 477-81. CrossRef

Denisova ON, Zhegunov GF, Hacbaui HA, Vachenko BB. [Efficiency of combined media application for bovine erythrocyte cryopreservation]. Scientific Bulletin of Lviv National Stepan Gzhytsky University of Veterinary Medicine and Biotechnology. 2013; 15(3): 71-7. Ukrainian.

Denisova ON, Zhegunov GF, Babijchuk LA. Cryopreservation of animal's erythrocytes under dimethyl sulfoxide, polyethyleneoxide and glycerol protection. Problems of Cryobiology 2005; 15(2): 195-201.

Elliott GD, Fuller BJ., Wang S. Cryoprotectants: A review of the actions and applications of cryoprotective solutes that modulate cell recovery from ultra-low temperatures. Cryobiology. 2017; 76: 74-91. CrossRef

Florin-Christensen J, Suarez CE, Florin-Christensen MA. Unique phospholipid organization in bovine erythrocyte membranes. PNAS. 2001; 98(14): 7736-41. CrossRef

Lahmann JM, Benson JD, Higgins AZ. Concentration dependence of the cell membrane permeability to cryoprotectant and water and implications for design of methods for postthaw washing of human erythrocytes. Cryobiology. 2018; 80; 1-11. CrossRef

Leibo SP. Freezing damage of bovine erythrocytes: simulation using glycerol concentration changes at subzero temperatures. Cryobiology. 1976; 13(6); 587-98. CrossRef

Liu L, Lei T, Bankir L, et al. Erythrocyte permeability to urea and water: comparative study in rodents, ruminants, carnivores, humans, and birds. J Comp Physiol B. 2011; 181(1): 65-72. CrossRef

Obrador R, Musulin S, Hansen BJ. Red blood cell storage lesion. J Vet Emerg Crit Care. 2015; 25(2): 187-99. CrossRef

Pogozhykh D, Pakhomova Y, Pervushina O, et al. Exploring the possibility of cryopreservation of feline and canine erythrocytes by rapid freezing with penetrating and non-penetrating cryoprotectants. PLoS One [Internet]. 2017 [Cited Jan 10, 2018];12(1): 1-16. Available from: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0169689. CrossRef

Rozanski E, de Laforcade AM. Transfusion medicine in veterinary emergency and critical care medicine. Clin Tech Small Anim Pract. 2004; 19(2): 83-7. CrossRef

Shpakova NM, Orlova NV, Nipot EE, et al. [Osmotic sensitivity of mammalian erythrocytes under their initial state modification]. Visnyk Problem Biol Med. 2016; (3, Pt 2): 356-61. Russian.

Published

2019-09-19

How to Cite

Nipot, O. Y., Yershova, N. A., Shapkina, O. O., Yershov, S. S., & Kuchkov, V. M. (2019). Glycerol Impact on Human and Bovine Erythrocytes Under Hypertonic Shock. Problems of Cryobiology and Cryomedicine, 29(3), 199-205. https://doi.org/10.15407/cryo29.03.199

Issue

Section

Theoretical and Experimental Cryobiology