Glycerol Impact on Human and Bovine Erythrocytes Under Hypertonic Shock

Authors

  • Olena Ye. Nipot Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Natalya A. Yershova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Olga O. Shapkina Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Sergiy S. Yershov Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Vadim M. Kuchkov Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

DOI:

https://doi.org/10.15407/cryo29.03.199

Keywords:

mammalian erythrocytes, hypertonic shock, penetrating cryoprotectant, glycerol, incubation temperature, hemolysis

Abstract

Here, we have studied the impact of preliminary incubation of human and bovine erythrocytes in glycerol solutions on their sensitivity to hypertonic shock. The cell damage was shown to significantly reduce with increasing the glycerol concentration. The ranges of a sharp change in human and bovine cell sensitivity were from 0.1 to 0.5 and 0.5 to 1 M, respectively. A change in incubation time with glycerol from 1 to 10 min resulted in sensitivity decrease to hypertonic shock for both mammalian species, but the curves of dependence were different. A damaging effect of hypertonic shock increased with temperature rise from 0 up to 10°C for human erythrocytes and from 0 to 25°C for bovine ones. Preliminary erythrocyte incubation with glycerol entailed a decreased hypertonic damage of erythrocytes at all the studied temperatures for human cells and at those above 5°C for bovine cells. Our findings and the reported data review suggested about a different protective mechanism for human and bovine erythrocytes using glycerol under hypertonic shock conditions.

 

Probl Cryobiol Cryomed 2019; 29(3):199–205.

Author Biographies

Olena Ye. Nipot, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryocytology

Natalya A. Yershova, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryocytology

Olga O. Shapkina, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryocytology

Sergiy S. Yershov, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryocytology

Vadim M. Kuchkov, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryocytology

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Published

2019-09-19

How to Cite

Nipot, O. Y., Yershova, N. A., Shapkina, O. O., Yershov, S. S., & Kuchkov, V. M. (2019). Glycerol Impact on Human and Bovine Erythrocytes Under Hypertonic Shock. Problems of Cryobiology and Cryomedicine, 29(3), 199–205. https://doi.org/10.15407/cryo29.03.199

Issue

Section

Theoretical and Experimental Cryobiology