Study of Interaction of Glycerol Cryoprotectant and Its Derivatives with Dimethylacetamide in Aqueous Solution Using Fluorescent Probes

Authors

  • Tetiana S. Dyubko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv https://orcid.org/0000-0003-3207-4779
  • Vasyl G. Pivovarenko Taras Shevchenko National University of Kyiv, Kyiv
  • Valentina V. Chekanova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Yuliya S. Pakhomova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv https://orcid.org/0000-0003-2682-3796
  • Yana V. Hvozdiuk Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Antonina M. Kompaniets Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Anatoliy L. Tatarets Institute for Single Crystals of the National Academy of Sciences of Ukraine, Kharkiv https://orcid.org/0000-0003-4406-7883

DOI:

https://doi.org/10.15407/cryo31.02.139

Keywords:

glycerol, oxyethylated derivatives, dimethylacetamide, cryoprotectants, mixtures, membranotropy, 3-hydroxy-4´- (N, N dimethylaminoflavone), fluorescence

Abstract

In this paper we have studied the interaction of the mixtures of glycerol (GL) and its oxyethylated derivatives (OEG) with polymerization degree n = 3, 25 and 30 with dimethylacetamide (DMAc) in aqueous solution using 3-hydroxy-4´-(N, N dimethylaminoflavone) fluorescent probe. The combination of GL and its oxyethylated derivatives with DMAc was found to reduce the membranotropy of certain cryoprotective agents, forming a mixture. The combination of both GL and its low molecular weight derivative (OEGn=3) with DMAc reduced the membranotropy of the latter. At the same time, combining GL derivatives of high molecular weight (OEGn=25 and OEGn=30) with DMAc diminished the membranotropy of OEG. The OEGn=30 at concentrations above 1 wt.% was shown to form the micellar-type structures or micellar associates in aqueous solution. This enabled suggesting the membranotropic ability of high molecular weight OEG associates to be stipulated by possible interaction of their nonpolar segments with nonpolar sites on biomembrane surface. Structural rearrangements of molecular associates in aqueous solutions of low and high molecular weight cryoprotectant mixtures were designated as the experimentally established mechanism of cytotoxicity reduction in combined cryoprotective media.

Probl Cryobiol Cryomed 2021; 31(2): 139–150

 

Author Biographies

Tetiana S. Dyubko , Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Laboratory of Cryoprotectants

Valentina V. Chekanova , Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Laboratory of Cryoprotectants

Yuliya S. Pakhomova , Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Laboratory of Cryoprotectants

Yana V. Hvozdiuk, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Laboratory of Cryoprotectants

Antonina M. Kompaniets, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Laboratory of Cryoprotectants

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Published

2021-07-15

How to Cite

Dyubko, T., Pivovarenko, V., Chekanova, V., Pakhomova, Y., Hvozdiuk, Y., Kompaniets, A., & Tatarets, A. (2021). Study of Interaction of Glycerol Cryoprotectant and Its Derivatives with Dimethylacetamide in Aqueous Solution Using Fluorescent Probes. Problems of Cryobiology and Cryomedicine, 31(2), 139–150. https://doi.org/10.15407/cryo31.02.139

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Section

Theoretical and Experimental Cryobiology