Thermoplastic Analysis of Cluster Crystallization of Cryoprotective Solutions

Authors

  • Oleksandr I. Osetskyi Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Tetyana M. Gurina Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Anna L. Poliakova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Stanislav S. Sevastianov Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Keywords:

thermoplastic deformation, state diagram, cluster crystallization, splitting, cryoprotectants

Abstract

For the first time the possibility of using thermomechanical analysis to construct the fragments of state diagrams of cryoprotective solutions in the zone of glass transition temperatures has been considered. A method for studying cluster crystallization of cryoprotective solutions based on thermomechanical curves has been developed. The parameters of thermomechanical curves of frozen aqueous solutions of dimethyl sulfoxide (DMSO), polyethylene oxide 1500 (PEO-1500), glycerol were analyzed and the relationship between these parameters and the cluster crystallization kinetics for these solutions was established. On the basis of experimentally obtained thermomechanical curves for the frozen solutions of DMSO and PEO-1500 the possibility of formation of clusters of two types has been shown: on the basis of ice and cryoprotective substance microcrystals. Additional experimental data were obtained to construct a complete state diagrams of cryoprotective solutions, which include the existing cluster phase areas.

 

Probl Cryobiol Cryomed 2021; 31(3): 203–213

Author Biographies

Oleksandr I. Osetskyi, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Laboratory of Cryoprotectants

Tetyana M. Gurina, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryomicrobiology

Stanislav S. Sevastianov , Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryomicrobiology

References

Abidalla M, Roversi PF. Vitrification Assessment: Thermal analysis of cryoprotective aqueous solutions 1,2 propanediol and ethylene glycol. Biopreservation and Biobanking. 2018; 16 (3): 207–16.

Boutron P, Kaufmann A. Stability of the amorphous state in the system water – glycerol – dimethylsulphoxide. Cryobiology. 1978; 15 (1): 93–108.

Cocks FA, Brower WE. Phase diagram relationships in cryobiology. Cryobiology. 1974; 1 (4): 340–58.

Flores-Ramirez AJ, Garcia-Coronado P, Grajales-Lagunes A, Gonzalez R. Freeze-concentrated phase and state transition temperatures of mixtures of low and high molecular weight cryoprotectants. Advances in Polymer Technology. 2019; 1–11.

Gosh RC, Toda A, Tanaka S. The effect of NaCl on the eutectic phase behavior of aqueous poly (ethylene glycol) solutions. Polymer. 2009; 50 (5): 1304–10.

Hey JM, MacFarlane DR. Kinetic analyses of crystallization and devitrification: Comparison of activation energies in aqueous solutions of glycerol and dimethyl sulphoxide. Journal of Non Crystalline Solids. 1997; 211 (3): 262–70.

Kanno H, Kajiwarw K, Miyata K. Supercooling of aqueous dimethylsulfoxide solution at normal and high pressures: evidence for the coexistence of phase-separated aqueous dimethylsulfoxide solutions of different water structures. Journal of Chemical Physics. 2010; 132 (19): 194503-1–194503-8.

Kukhling Kh. [Physics reference]. Moscow: Mir; 1985. 520p. Russian.

MacFarlane DR. Devitrification in glass-forming aqueous solutions. Cryobiology. 1986; 23 (3): 230–44.

Moiseev VA, Mank VV, Zinchenco VD, et al. [Complexing properties of polyethylene glycols]. In: [Current Problems of Cryobiology and Cryomedicine]. Moscow: Vneshtorgizdat; 1975. 42–8. Russian.

Moiseev VA, Zinchenco VD, Mank VV, et al. [Investigation of aqueous mixtures of polyethylene glycols by NMR]. Kolloidnyi Zhurnal. 1977; 39 (1): 30–5. Russian.

Mullen S, Critser J. The Science of Cryobiology. In: Woodruff T, Snyder K, editors. Oncofertility fertility preservation for cancer survivors. Part of the Cancer treatment and research book series (CTAR, volume 138). Boston: Springer; 2007. p. 83–109.

Murata К, Tanaka H. Liquid – liquid transition without macroscopic phase separation in a water – glycerol mixture. Nature Materials. 2012; 11 (5): 436–43.

Osetsky АI, Kirilyuk AL, Gurina TM. Study of devitrification kinetics of cryoprotectant aqueous solutions using thermoplastic deformation method. Problems of Cryobiology. 2005; 15 (2): 137–46.

Osetsky АI. Peculiarities of state diagrams of aqueous solutions of cryoprotective agents. Cryobiology. 2009; 59, (2): 141–9.

Osetsky АI. Тhermodynamic aspects of cluster crystallization in cryoprotective solutions. CryoLetters. 2011; 32 (3): 216–24.

Osetsky АI. To theory of diagrams of state for cryoprotective solutions. Probl Cryobiol Cryomed. 2014; 24 (2): 102–17.

Osetsky АI, Sevastyanov SS. Effect of hydrogen bonds on crystallization kinetics of aqueous solutions of cryoprotective agents. Probl Cryobiol Cryomed. 2016; 26 (3): 199–212.

Rasmussen DH, MacKenzie AP. Phase diagram for the system water – dimethylsulphoxide. Nature. 1968; 220: 1315–7.

Teitelbaum BY. [Thermomechanical analysis of polymers]. Moscow: Nauka; 1979. 236 p. Russian.

Wendlandt W. Thermal methods of analysis. New York: Wiley; 1974. 505 p.

Wowk B. Thermodynamic aspects of vitrification. Cryobiology. 2010; 60 (1): 11–22.

Zinchenco AV, Вobrova EN, Schetinskiy MI. [Glycerol and 1,2 propanediol effect on phase transitions and vitrification in cord blood erythrocyte suspension under 0°C]. Dopovidi NAN Ukrainy. 2003; (12): 155–60. Russian.

Zinchenco AV, Вobrova EN, Schetinskiy MI. DMSO effect on phase transitions and vitrification in cord blood erythrocyte suspension under 0°C. Problems of Cryobiology. 2003; (2): 16–21.

Zinchenco AV, Mank VV, Moiseev VA. [About phase transitions and physical states of water-propanediol system]. Doklady Akademiy Nauk SSSR. 1983; 269 (1): 144–6. Russian.

Zhivotova EN, Zinchenko AV, Kuleshova LG, Chekanova VV, Kompaniets AM. Physical states of aqueous solutions of oxyethylated glycerol with polymerization degree of n = 30 at temperatures lower than 283 K. CryoLetters. 2007; 28 (4): 261–70.

Downloads

Published

2021-10-08

How to Cite

Osetskyi, O., Gurina, T., Poliakova , A., & Sevastianov , S. (2021). Thermoplastic Analysis of Cluster Crystallization of Cryoprotective Solutions. Problems of Cryobiology and Cryomedicine, 31(3), 203–213. Retrieved from http://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/1739

Issue

Section

Theoretical and Experimental Cryobiology