Cryopreservation as Biotechnological Application of Dendritic Cells in Clinical Practice

Authors

  • Anatoliy М. Goltsev Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv; Interdepartmental Scientific Center of the National Academy of Sciences of Ukraine, Academy of Medical Sciences of Ukraine and Ministry of Health of Ukraine, Kharkiv https://orcid.org/0000-0002-5289-5876
  • Katerina Ye. Yampolska Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv https://orcid.org/0000-0002-5138-3385
  • Hanna G. Kisielova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Maksym V. Оstankov Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv https://orcid.org/0000-0001-8412-5262
  • Tatiana G. Dubrava Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv https://orcid.org/0000-0002-1056-5107
  • Natalya М. Babenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv https://orcid.org/0000-0001-7002-0267
  • Yuliya О. Gaevska Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv https://orcid.org/0000-0002-2375-357X
  • Mykola О. Bondarovych Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv https://orcid.org/0000-0001-7977-0283

DOI:

https://doi.org/10.15407/cryo31.04.289

Keywords:

cryopreservation, mononuclear cells, monocytes, tolerogenic dendritic cells, autoimmune diseases

Abstract

An important component of the pathogenesis of autoimmune diseases is the immune system deregulation as an impaired tolerance to its own antigens by reducing the content of T-regulatory cells. Their formation is closely related to the function of dendritic cells (DCs), so in autoimmune diseases the use of DCs with tolerogenic potential is promising for the restoration of antigen-specific tolerance. Recently, the issue of establishing the banks of tolerogenic DCs for clinical use, which involves their cryopreservation, has been actively discussed. To date, there is no common  protocol for DCs freezing, which would take into account the different sources of their obtaining,  the initial structural and functional state before freezing, composition of cryopreservation media and other factors. The review summarizes experimental data on cryopreservation of peripheral blood and bone marrow mononuclear cells or monocytes. The potential for their further ex vivo differentiation into  DCs to ensure the stability of immature phenotype and tolerogenic function has been studied.

Probl Cryobiol Cryomed 2021; 31(4): 289–303

Author Biographies

Anatoliy М. Goltsev , Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv; Interdepartmental Scientific Center of the National Academy of Sciences of Ukraine, Academy of Medical Sciences of Ukraine and Ministry of Health of Ukraine, Kharkiv

Department of Cryopatophysiology and Immunology

Katerina Ye. Yampolska, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryopatophysiology and Immunology

Hanna G. Kisielova, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryopatophysiology and Immunology

Maksym V. Оstankov , Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryopatophysiology and Immunology

Tatiana G. Dubrava , Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryopatophysiology and Immunology

Natalya М. Babenko, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryopatophysiology and Immunology

Yuliya О. Gaevska, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryopatophysiology and Immunology

Mykola О. Bondarovych, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryopatophysiology and Immunology

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Published

2021-12-24

How to Cite

Goltsev , A., Yampolska, K., Kisielova, H., Оstankov M., Dubrava , T., Babenko, N., Gaevska, Y., & Bondarovych, M. (2021). Cryopreservation as Biotechnological Application of Dendritic Cells in Clinical Practice. Problems of Cryobiology and Cryomedicine, 31(4), 289–303. https://doi.org/10.15407/cryo31.04.289