Effect of Hypothermic Storage and Cryopreservation on Human Cord Blood Leucoconcentrate Cells
Keywords:human cord blood, stem hemopoietic and non-hemopoietic cells, hypothermic storage, cryopreservation
Pre-cryopreservation hypothermic storage and cryopreservation process itself effects on integrity, viability and membrane properties of hemopoietic stem cells with high proliferative activity and self-support potentials as well as of non-hemopoietic human cord blood leucoconcentrate (HCBL) cells were investigated, as well as correlation between phenotypic marker expression and functional condition of these cells was assessed. The data obtained allowed experimental substantiating the maximally acceptable term of hypothermic storage (24 hrs) of cord blood prior to production of HCBL. A rise in plasmatic membrane permeability of stem hemopoietic cells with a high proliferative activity potential after their cryopreservation was shown. The permeability also increased, as the term of preliminary hypothermic storage was prolonged.
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Copyright (c) 2020 A. N. Goltsev, V. V. Volina, L. V. Sokol, M. V. Ostankov, K. A. Goltsev, S. S. Chernousova, O. Yu. Kozhina
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