Effect of Hypothermic Storage and Cryopreservation on Human Cord Blood Leucoconcentrate Cells

Authors

  • A. N. Goltsev Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • V. V. Volina Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • L. V. Sokol Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • M. V. Ostankov Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • K. A. Goltsev Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • S. S. Chernousova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • O. Yu. Kozhina Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

human cord blood, stem hemopoietic and non-hemopoietic cells, hypothermic storage, cryopreservation

Abstract

Pre-cryopreservation hypothermic storage and cryopreservation process itself effects on integrity, viability and membrane properties of hemopoietic stem cells with high proliferative activity and self-support potentials as well as of non-hemopoietic human cord blood leucoconcentrate (HCBL) cells were investigated, as well as correlation between phenotypic marker expression and functional condition of these cells was assessed. The data obtained allowed experimental substantiating the maximally acceptable term of hypothermic storage (24 hrs) of cord blood prior to production of HCBL. A rise in plasmatic membrane permeability of stem hemopoietic cells with a high proliferative activity potential after their cryopreservation was shown. The permeability also increased, as the term of preliminary hypothermic storage was prolonged.

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Published

2010-09-20

How to Cite

Goltsev, A. N., Volina, V. V., Sokol, L. V., Ostankov, M. V., Goltsev, K. A., Chernousova, S. S., & Kozhina, O. Y. (2010). Effect of Hypothermic Storage and Cryopreservation on Human Cord Blood Leucoconcentrate Cells. Problems of Cryobiology and Cryomedicine, 20(3), 303–308. Retrieved from http://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/190

Issue

Vol. 20 No. 3 (2010): Problems of Cryobiology

Section

Cryopreservation of Biological Resources

License

Copyright (c) 2020 A. N. Goltsev, V. V. Volina, L. V. Sokol, M. V. Ostankov, K. A. Goltsev, S. S. Chernousova, O. Yu. Kozhina

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