Effect of Dimethyl Sulfoxide and Diethyl Sulfoxide on Thermal Denaturation of Human Serum Albumin
Keywords:albumin, thermal denaturation, electron spectroscopy, fluorescent spectroscopy, dimethyl sulfoxide, diethyl sulfoxide
The effect of dimethyl sulfoxide (DMSO) and diethyl sulfoxide (DESO) on thermal (heat and cold) denaturation of human serum albumin within temperature range from â€“20 to90Â°Cwas investigated by electron and fluorescent spectroscopy. It has been established that at heat induced denaturation in the presence of DESO an acid-neutral transition was observed at 47.61Â°C. It was shown that at cold induced denaturation the direct hydrophobic interactions of sulphoxide molecules with protein surface result in the formation of stable â€œiceâ€ layer, providing the protein protection from irreversible structure changes.
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Copyright (c) 2020 K. R. Grigoryan, S. A. Markarian, M. G. Aznauryan
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