Effect of keeping the tissue architecture on adrenal gland cryosensitivity in mice and newborn piglets

Authors

  • V. D. Ustichenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • N. M. Alabedalkarim Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • T. P. Bondarenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

adrenocortical cells, organ culture, cryopreservation, basal secretion, stimulated secretion

Abstract

We investigated the influence of keeping the histological architecture on the cryosensitivity of single cells suspension and fragments of mice and newborn piglets’ adrenal glands. It was shown that the adrenal gland fragments were more resistant to effect of low temperature preservation factors, comparing to single adrenocortical cells, and as a result, had both basal and stimulated steroidogenesis. The highest diffusion barrier in fragments changes their sensitivity to effect of stimulators, efficient at the interaction with intracellular targets, but doesn’t influence the regulation, which are realized due binding with receptors, dirrected to the cell outside.

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Published

2004-09-15

How to Cite

Ustichenko, V. D., Alabedalkarim, N. M., & Bondarenko, T. P. (2004). Effect of keeping the tissue architecture on adrenal gland cryosensitivity in mice and newborn piglets. Problems of Cryobiology and Cryomedicine, (3), 39–45. Retrieved from http://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/673

Issue

No. 3 (2004): Problems of Cryobiology

Section

Theoretical and Experimental Cryobiology

License

Copyright (c) 2020 V. D. Ustichenko, N. M. Alabedalkarim, T. P. Bondarenko

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