Effect of keeping the tissue architecture on adrenal gland cryosensitivity in mice and newborn piglets
Keywords:adrenocortical cells, organ culture, cryopreservation, basal secretion, stimulated secretion
We investigated the influence of keeping the histological architecture on the cryosensitivity of single cells suspension and fragments of mice and newborn pigletsâ€™ adrenal glands. It was shown that the adrenal gland fragments were more resistant to effect of low temperature preservation factors, comparing to single adrenocortical cells, and as a result, had both basal and stimulated steroidogenesis. The highest diffusion barrier in fragments changes their sensitivity to effect of stimulators, efficient at the interaction with intracellular targets, but doesnâ€™t influence the regulation, which are realized due binding with receptors, dirrected to the cell outside.
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Copyright (c) 2020 V. D. Ustichenko, N. M. Alabedalkarim, T. P. Bondarenko
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