Effect of Trifluoperazine and Dodecyl-β,D-maltoside on Hypertonic Stress of Mammalian Erythrocytes

Authors

  • Natalia A. Yershova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • Elena E. Nipot Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • Natalia M. Shpakova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • Sergey S. Yershov Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • Natalia V. Orlova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

DOI:

https://doi.org/10.15407/cryo24.03.231

Keywords:

mammalian erythrocytes, hypertonic stress, amphiphilic compounds, potassium cations

Abstract

The effect of dodecyl-β,D-maltoside (DM) and trifluoperazine (TFP) on mammalian (human, bovine, equine, rabbit)
erythrocyte sensitivity to hypertonic stress (HS) (4.0 mol/l NaCl) was studied in this work. The use of amphiphilic compounds in the certain concentrations was demonstrated as resulting in an increased resistance to HS of human, bovine, equine erythrocytes, but not rabbit ones. Protective effect of amphiphilic compounds is higher at 37°C than at 0°C. Cationic TFP is more efficient than non-ionic DM. The substances under study were established as not affecting the potassium cation release out of cells under HS. Protective mechanism of TFP and DM is suggested as associated with their perturbating action on a membrane and prevention of transmembrane defect development up to hemolytic pore size.


Probl Cryobiol Cryomed 2014; 24(3):231-237.

Author Biographies

Natalia A. Yershova, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Department of Cell Cryophysiology

Elena E. Nipot, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Department of Cell Cryophysiology

Natalia M. Shpakova, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Department of Cell Cryophysiology

Sergey S. Yershov, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Department of Cell Cryophysiology

Natalia V. Orlova, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Department of Cell Cryophysiology

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Published

2014-09-15

How to Cite

Yershova, N. A., Nipot, E. E., Shpakova, N. M., Yershov, S. S., & Orlova, N. V. (2014). Effect of Trifluoperazine and Dodecyl-β,D-maltoside on Hypertonic Stress of Mammalian Erythrocytes. Problems of Cryobiology and Cryomedicine, 24(3), 231–237. https://doi.org/10.15407/cryo24.03.231

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Section

Theoretical and Experimental Cryobiology