Effect of Trifluoperazine and Dodecyl-β,D-maltoside on Hypertonic Stress of Mammalian Erythrocytes

Natalia A. Yershova, Elena E. Nipot, Natalia M. Shpakova, Sergey S. Yershov, Natalia V. Orlova

Abstract


The effect of dodecyl-β,D-maltoside (DM) and trifluoperazine (TFP) on mammalian (human, bovine, equine, rabbit)
erythrocyte sensitivity to hypertonic stress (HS) (4.0 mol/l NaCl) was studied in this work. The use of amphiphilic compounds in the certain concentrations was demonstrated as resulting in an increased resistance to HS of human, bovine, equine erythrocytes, but not rabbit ones. Protective effect of amphiphilic compounds is higher at 37°C than at 0°C. Cationic TFP is more efficient than non-ionic DM. The substances under study were established as not affecting the potassium cation release out of cells under HS. Protective mechanism of TFP and DM is suggested as associated with their perturbating action on a membrane and prevention of transmembrane defect development up to hemolytic pore size.


Probl Cryobiol Cryomed 2014; 24(3):231-237.


Keywords


mammalian erythrocytes; hypertonic stress; amphiphilic compounds; potassium cations

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DOI: https://doi.org/10.15407/cryo24.03.231

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