Correction of Anemia Resulting From 5-Fluorouracil Toxic Effect Using Cryopreserved Preparations of Cord Blood

Olga V. Kudokotseva, Igor F. Kovalenko, Ivan I. Lomakin, Georgiy A. Babijchuk

Abstract


The use of the most chemotherapeutic drugs leads to the development of hypoplasia of bone marrow hematopoiesis and severe anemia. The effect of cryopreserved preparations of human cord blood (HCB) (cellular and acellular ones) on the reco-very of erythroid elements in peripheral blood (PB) was studied in the model of cytostatic hemodepression with 5-fluorouracil (5-FU) in mice. An intravenous administration of cryopreserved nucleated cells (cNCs) of HCB was shown as contributed to an accelerated recovery of the absolute number of erythrocytes at the earlier terms than in the groups of comparison (5-FU and 5-FU + cell-free HCB supernatant). There was established, that to day 9 after administering HCB cNCs the erythrocyte number in murine PC was completely recovered and their outer contour was kept.


Probl Cryobiol Cryomed 2014; 24(4):312-321.


Keywords


cord blood; chemotherapy; anemia; erythrocytes; sphericity index; hemolysis

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DOI: https://doi.org/10.15407/cryo24.04.312

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