Histological Picture in Neocortex and Hypothalamus of Homoio- and Heterothermal Animals Under Artificial and Natural Hypometabolism
Keywords:neocortex, hypothalamus, dark neurons, hibernation, artificial hypometabolic state, homoiothermia, heterothermia
Structural changes in the brain neocortex and hypothalamus in heterothermal hamsters under natural and artificial hypometabolism, and in homoiothermal rats under artificial hypometabolism as well as 2 and 24 hrs later awakening from hypometabolic states were studied. The following changes in the studied brain tissues were observed: a dilation/constriction of perivascular spaces and dark neurons appearance; the emergence of neurons with nucleoli in their nucleus, likely indicating an increased protein synthesis in response to hypoxia. The latter was confirmed by the revealed corresponding fluctuations in the total protein levels in the tissues. The identified changes probably reflect different phases of normal metabolic processes under hypometabolism and are not resulted from either toxic effect or pathological manifestations. The changes were species-specific and manifested differently, both qualitatively and quantitatively, at certain stages of entering and awakening from hypometabolic states.
Probl Cryobiol Cryomed 2015; 25(2): 93-103
Beckman A.L., Stanton T.L. Properties of the CNS during the state of hibernation. The Neural Basis of Behavior. NY: MPTP press; 1982.
Bradford M.M. A Rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976; 72 (7): 248â€“254.
Capo I., Lalosevic D. Interpretation of dark neurons in experimental model of ischemia, neurointoxication and brain infection. Med Pregl 2011; 64 (1â€“2): 101â€“106.
Cortez S.C., McIntosh T.K., Noble L.J. Experimental fluid percussion brain injury: vascular disruption and neuronal and glial alterations. Brain Res 1989; 482 (2): 271â€“282. CrossRef
Csordas A., Mazlo M., Gallyas F. Recovery versus death of "dark" (compacted) neurons in non-impaired parenchymal environment: light and electron microscopic observations. Acta Neuropathol 2003; 106 (1): 37â€“49.
Deveci D., Egginton S. Differing mechanisms of cold-induced changes in capillary supply in m. tibialis anterior of rats and hamsters. J Experim Biology 2002; 205 (Pt. 6): 829â€“840.
Drew K.L., Buck C.L., Barnes B.M. et al. Central nervous system regulation of mammalian hibernation: implications for metabolic suppression and ischemia tolerance. J Neurochem 2007; 102 (6): 1713â€“1726. CrossRef PubMed
Drew K.L., Rice M.E., Kuhn T.B., Smith M.A. Neuroprotective adaptations in hibernation: therapeutic implications for ische-mia-reperfusion, traumatic brain injury and neurodegenerative diseases. Free Radic Biol Med 2001; 31 (5): 563â€“573. CrossRef
Frerichs K.U., Smith C.B, Brinner M. et al. Suppression of protein synthesis in brain during hibernation involves inhibition of protein initiation and elongation. Proc Natl Acad Sci USA 1998; 95 (11): 14511â€“14516. CrossRef PubMed
Gallyas F., Zoltay G. An immediate light microscopic response of neuronal somata, dendrites and axons to noncontusing concussive head injury in the rat. Acta Neuropathol. 1992; 83 (5): 386â€“393.
Gallyas F., Zoltay G., Balas I. An immediate light microscopic response of neuronal somata, dendrites and axons to contusing concussive head injury in the rat. Acta Neuropathol 1992; 83 (5): 394â€“401. CrossRef PubMed
Garman R.H. Histology of the central nervous system. Toxicol Pathol 2011; 39 (5): P. 22â€“35.
Himms-Hagen S. Brown adipose tissue and cold-acclimation. In: P. Trayhurn and D.G. Nicholls, editors. Brown Adipose Tissue. London: Edward Arnold Ltd, 1986. p. 214â€“267.
Klimenko Ðž.A., Rogachevsky V.V. Volumetric ultrastructure of dendritic synapses of identified light and dark rats hippocampal neurons. Proceedings of XXIV Russian Conf. on Electron Microscopy (RKEM 2012); Chernogolovka, 2012: 427â€“428.
Kryzhanovsky G.N. General pathophysiology of nervous system: Handbook. Ðœoscow: Medicine; 1997.
Larson J., Drew K.L., Folkow L.P. et al. No oxygen? No problem! Intrinsic brain tolerance to hypoxia in vertebrates. J Exp Biol 2014; 217 (Ð t. 7): 1024â€“1039.
Lowenstein D.H., Thomas M.J., Smith D.H., McIntosh T.K. Selective vulnerability of dentate hilar neurons following traumatic brain injury: a potential mechanistic link between head trauma and disorders of the hippocampus. J Neurosci 1992; 12 (12): 4846â€“4853.
Ma Y.L., Zhu X., Rivera P. et al. Absence of cellular stress in brain after hypoxia induced by arousal from hibernation in Arctic ground squirrels. Am J Physiol Regul Integr Comp Physiol. 2005; 289: R1297â€“R1306.
Magarinos A.M., McEwen B.S., Saboureau M., Pevet P. Rapid and reversible changes in intrahippocampal connectivity during the course of hibernation in European hamsters. PNAS 2006; 103 (49): 18775â€“18780. CrossRef PubMed
Melnichuk Ð¡.D., ÐœÐµlnichuk D.Ðž. Hypobios of animals (molecular mechanisms and practical value for agriculture and medicine). Kyiv: Vydavnytstvo NAU; 2007.
Morrison S.F., Nakamura K. Central neural pathways for ther-moregulation. Front Biosci 2011; 16 (1): 74â€“104. CrossRef
Murakami T., Ohtsuka A. Dark neurons in the mouse brain: An investigation into the possible significance of their variable appearance within a day and their relation to negatively charged cell coats. Arch Histol Cytol 1996; 59 (1): 79â€“85. CrossRef
Murakami T., Ohtsuka A., Taguchi T., Piao D.X. Perineuronal sulfated proteoglicans and dark neurons in the brain and spinal cord: A histochemical and electron microscopic study of new-born and adult mice. Arch Histol Cytol 1995; 58 (5): 557â€“565. CrossRef PubMed
Nawashiro H., Shima K., Chigasaki H. Selective vulnerability of hippocampal CA3 neurons to hypoxia after mild concussion in the rat. Neurol Res 1995; 17 (6): 455â€“460.
Ooigawa H., Nawashiro H., Fukui S. et al. The fate of Nissl-stained dark neurons following traumatic brain injury in rats: difference between neocortex and hippocampus regarding survival rate. Acta Neuropathol 2006; 112 (4): 471â€“481. CrossRef PubMed
Pastukhov Yu.F., ÐœÐ°ksimov Ð.L., Khaskin Ð’.Ð’. Adaptation to cold and subarctic conditions: Problems of thermal physiology. ÐœÐ°gadan: NESC FEB RAS, 2003; 1.
Polenov L.Ð. Functional morphology of Gomori-positive hypo-thalamic-pituitary neurosecretory system during the winter torpor in poikilothermal and homoiothermal vertebrates from hibernation. Cryobiology and Cryomedicine 1984; (15): 44â€“47.
Shilo O.V. Dynamics of electrographic indices in rats and hamsters under artificial and natural hypometabolic states. Neurofiziologiya 2015; 47 (1): 87â€“95.
Shtark Ðœ.Ð’. Brain of hibernators. Novosibirsk: Nauka; 1970.
Timofeev N.N., Prokofieva L.P. Neurochemistry of hypobiosis and limits of organisms cryoresistance. Ðœoscow: Medicine; 1997.
Volkova Ðž.V., Eletskiy Yu.K. Bases of histology and histological techniques. Moscow: Meditsyna; 1982.
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