Craniocerebral Hypothermia Stimulates Reactions of Limited Proteolysis in Rat Tissues

Authors

  • Viktoriya V. Lomako Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Aleksandr V. Shylo Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Georgiy A. Babijchuk Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Lyubov M. Samokhina L.T. Malaya National Institute of Therapy of the National Academy of Medical Sciences of Ukraine, Kharkiv

DOI:

https://doi.org/10.15407/cryo26.03.238

Keywords:

craniocerebral hypothermia, total proteinases activity, non-trypsin-like proteinases, a-1-proteinases inhibitor, a-2-macroglobulin, rat

Abstract

The effect of moderate craniocerebral hypothermia (CCH) (32°C) on total proteinase activity (TPA), the activity of non-trypsin-like proteinases (NTLP) and their inhibitors (a-1-proteinase inhibitor (a-1-PI) and a-2-macroglobulin (a-2-MG) in the tissue samples of CNS (brain cortex, hypothalamus, cerebellum), peripheral organs (heart, lungs, liver, kidneys) and serum blood in rats was studied. In all the samples studied a sharp increase in the TPA (by one to two orders) and the NTLP activity (in several times) under CCH were observed. At the same time, against the background of constant a-1-PI activity the dynamics of the a-2-MG activity were multidirectional and tissue-specific: it was increased in blood serum, hypothalamus and heart, decreased in kidneys and cerebellum, and did not change in other tissues. Twenty four hours  after the CCH application, the TPA remained at the same high activity level, the NTLP activity returned to baseline levels in the most samples, the activity of a-1-PI decreased only in the brain cortex and hypothalamus; the a‑2‑MG activity decreased in hypothalamus, cerebellum and kidneys, increased in liver and heart and returned to the baseline levels in other samples. The sharp proteinases activation, shift of the dynamic balance between enzymes and their inhibitors under CCH and in the recovery phase may indicate crucial changes in the orgamism systems aimed at  implementation of  protective and therapeutic mechanisms.

 

Probl. Cryobiol. Cryomed. 2016; 26(3): 238–248

Author Biographies

Viktoriya V. Lomako, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryophysiology

Aleksandr V. Shylo, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryophysiology

Georgiy A. Babijchuk, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryophysiology

References

Alva N., Palomeque J., Carbonell T. Deep hypothermia protects against acute hypoxia in vivo in rats: a mechanism related to the attenuation of oxidative stress. Exp Physiol 2013; 98(6): 1115–1124. CrossRef PubMed

Alva N., Palomeque J., Carbonell T. Oxidative stress and antioxidant activity in hypothermia and rewarming: can RONS modulate the beneficial effects of therapeutic hypothermia? Oxidative Medicine and Cellular Longevity 2013; Article ID957054. CrossRef

Andryuschenko P.I. Subject of studying of cellular transplantology: metalloproteinases. Transplantologiya 2005; 8(1): 38–45.

Babijchuk G.A., Marchenko V.S., Lomakin I.I., Belostotskiy A.V. Neurophysiological processes of cooled brain. Kiev: Naukova dumka; 1992.

Garkavi L.Kh., Kvakina E.B., Ukolova M.A. Adaptive reactions and resistance of the organism. Rostov-on-Don; 1990. PubMed

Gasparetti A. L., de Souza C.T., Pereira-da-Silva M. et al. Cold exposure induces tissue-specific modulation of the insulin-signalling pathway in Rattus norvegicus. J Physiol 2003; 552(1): 149–162. CrossRef PubMed

Girotti M., Donegan J.J., Morilak D.A. Chronic intermittent cold stress sensitizes neuroimmune reactivity in the rat brain. Psychoneuroendocrinology 2011; 36(8): 1164–1174. CrossRef PubMed

Han H.S., Park J., Kim J.-H., Suk K. Molecular and cellular pathways as a target of therapeutic hypothermia: pharmacological aspect. Cur Neuropharmacol 2012; 10(1): 80–87. CrossRef PubMed

Kalenova I.E., Sharinova I.A., Shevelev O.A., Butrov A.V. An experience of clinical application of hypothermia in ischemic stroke treatment. Nevrologia, Neuropsikhiatria, Psikhosomatika 2012; (2): 41–44.

Kees H., Polderman M.D. Therapeutic hypothermia and cont-rolled normothermia in the intensive care unit: Practical consi-derations, side effects, and cooling methods. Crit Care Med 2009; 37(3): 1101–1120. CrossRef PubMed

Laborit H., Huguenard P., Collectif P. Pratigue de l`hiber-notherapie en chirurgie et en medecine. Editions Masson&Cie; 1956.

Lees M., Taylor D.J., Wooley D.E. Mast cell proteinases activate precursor forms of collagenase and stromelysin, but not of gelatinases A and B. Eur J Biochem 1994; 223(1): 171–177. CrossRef PubMed

Lomakin I.I., Kudokotseva O.V., Purysheva V.Yu. Therapeutic effect of cord blood preparation at an example of dermal structural changes under experimental hypothyreosis and its potentiation by aerocryotherapy. In: Panchenko O.A., editor. Cryotherapy: safe technology of application. Kiev: CPIC; 2012; 51–59.

Lomakin I.I., Shylo O.V., Kozlov O.V. et al. Potentiation of tissue therapy effect in the model of pathological aging of the brain in animals. Transplantologia 2000; 1(1): 270–271.

Lomako V.V., Samokhina L.M. Effect of rhythmic cooling on some ethological and biochemical indices in rats with experi-mental depression. Problems of Cryobiology 2011; 21(1): 22–33.

Lomako V.V., Shilo A.V., Lomako S.V., Babijchuk G.A. Etho-logical analysis of the combined application of cold and cell therapy in rats with reserpine model of depression. Problems of Cryobiology 2005: 15 (3): 471–472.

Lomako V.V., Shilo A.V., Lomako S.V., Babiychuk G.A. Cold influence and cord blood restore pattern of behavioral activity in stressed rats. Veterynarna medytsyna 2008; 89: 232–237.

Lyden P.D., Krieger D., Yenari M. et al. Therapeutic hypothermia for acute store. Int J Stroke 2006; 1(1): 9–19. CrossRef PubMed

Marchenko V.S., Babijchuk G.A., Lomako V.V. et al. General approach to the problem of increasing the permeability of hematoencephalic barrier and body cold resistance. Problems of Cryobiology 1994; (1): 24–32.

Meshalkin E.N., editor. Clinical physiology of artificial hypothermia. Novosibirsk: Nauka, 1997.

Samokhina L.M., Dubinin A.A., inventors. A method for deter-mining the activity of proteainses or their inhibitors in biological fluids. Patent of Ukraine Nr.20171, IPC C 12 Q 1/38. 1997 Dec. 25.

Samokhina L.M., Starodub N.F., Babijchuk G.A., Lomako V.V. Rhythmic cold effect on activity of elastases in female rats with alcohol-depended hypertension. Problems of Cryobiology 2012; 22(1): 49–60.

Savel'eva G.M., Shalina R.I., Smirnova A.A. et al. Asphyxia of full-term newborns. Complex therapy with the use of cranio-cerebral hypothermia. Akusherstvo i Ginekologiya 2015; (4): 19–24.

Shankaran S. Hypoxic-ischemic encephalopathy and novel strategies for neuroprotection. Clin Perinatol 2012; 39(4): 919–929. CrossRef PubMed

Turk E.E. Hypothermia. Forensic Sci Med Pathol 2010; 6(2): 106–115. CrossRef PubMed

Tu-Sekine B., Raben D.M. Dual regulation of diacylglycerol kinase (DGK). Polybasic proteins promote activation by phospholipids and increase substrate affinity. J Biol Chem 2012; 287(50): 41619–41627. CrossRef PubMed

Veremeyenko K.N., Goloborod`ko O.P., Kizim A.I. Proteolysis in norm and pathology. Kiev: Zdorovya; 1988.

Wang X., Che H., Zhang W. et al. Effects of mild chronic intermittent cold exposure on rat organs. Int J Biol Sci 2015; 11(10): 1171–1180. CrossRef PubMed

Xing J.-Q., Zhou Y., Chen J.-F. et al. Effect of cold adaptation on activities of relevant enzymes and antioxidant system in rats. Int J Clin Exp Med 2014; 7(11): 4232–4237. PubMed

Yenari M.A., Han H.S. Neuroprotective mechanisms of hypothermia in brain ischaemia. Neurosci 2012; 13(4): 267–278. CrossRef

Published

2016-09-23

How to Cite

Lomako, V. V., Shylo, A. V., Babijchuk, G. A., & Samokhina, L. M. (2016). Craniocerebral Hypothermia Stimulates Reactions of Limited Proteolysis in Rat Tissues. Problems of Cryobiology and Cryomedicine, 26(3), 238–248. https://doi.org/10.15407/cryo26.03.238

Issue

Section

Theoretical and Experimental Cryobiology