Cryopreserved Umbilical Cord Extract Restores the Skin Structure in Ovariectomized Rats
DOI:
https://doi.org/10.15407/cryo34.01.033Keywords:
umbilical cord, cryoextract, cryopreservation, rats, ovariectomy, stem cells, hyaluronic acid, aging, skinAbstract
The research covers the specific effect of human umbilical cord cryoextract and the culture medium of its mesenchymal stromal cells in comparison with synthetic hyaluronic acid on the skin regeneration in female rats with simulated aging. The specified substances were administered intradermally to ovariectomized animals. The injection of human umbilical cord cryoextract into animals with a model of skin aging was established to restore the skin structure already 2 weeks after the injections. Thickening of the epidermis and dermis, phenomenon of cell proliferation, and an increased number of collagen fibers as well as blood vessels were observed. Four weeks after the last injection, the skin condition was normalized to the level of intact animals. Administration of the culture medium of mesenchymal stromal cells of the umbilical cord also led to an increased number of collagen fibers, cell proliferation, contouring of skin layers, but had a less pronounced effect compared to the injections of cryoextract on the indices of the dermis and epidermis thickness. Injections of synthetic hyaluronic acid led to "rejuvenation" of the upper layers of skin and had a less pronounced manifestation compared to the effect of human umbilical cord derivatives.
Probl Cryobiol Cryomed 2024; 34(1):033–044
References
Abbaszadeh H, Ghorbani F, Derakhshani M, et al. Regenerative potential of Wharton's jelly-derived mesenchymal stem cells: A new horizon of stem cell therapy. J Cell Physiol. 2020; 235(12): 9230-40. CrossRef
Boismal F, Serror K, Dobos G, et al. Vieillissement cutané -Physiopathologie et thérapies innovantes. Med Sci (Paris). 2020; 36(12):1163-72. CrossRef
Deng Q, Huang S, Wen J, et al. PF-127 hydrogel plus sodium ascorbyl phosphate improves Wharton's jelly mesenchymal stem cell-mediated skin wound healing in mice. Stem Cell Res Ther [Internet]. 2020 Apr 3 [Cited 11.11.2022]; 11(1): 143. Available from: https://stemcellres.biomedcentral.com/articles/10.1186/s13287-020-01638-2 CrossRef
Farage MA, Miller KW, Elsner P, Maibach HI. Characteristics of the aging skin. Adv Wound Care. 2013; 2(1): 5-10. CrossRef
Fornieri C, Quaglino D Jr, Mori G. Correlations between age and rat dermis modifications. Ultrastructural-morphometric evaluations and lysyl oxidase activity. Aging Clin Exp Res. 1989; 1(2): 127-38. CrossRef
Gutowski KA. Hyaluronic acid fillers: Science and clinical uses. Clin Plast Surg. 2016; 43(3): 489-96. CrossRef
Juncan AM, Moisă DG, Santini A, et al. Advantages of hyaluronic acid and its combination with other bioactive ingredients in cosmeceuticals. Molecules [Internet]. 2021 Jul 22 [Cited 11.11.2022]; 26(15): 4429. Available from: https://www.mdpi.com/1420-3049/26/15/4429/htm CrossRef
Keen MA. Hyaluronic acid in dermatology. Skinmed. 2017; 15(6): 441-8. PubMed
Khavkin J, Ellis DA. Aging skin: histology, physiology, and pathology. Facial Plast Surg Clin. 2011; 19(2): 229-34.
Li T, Xia M, Gao Y, et al. Human umbilical cord mesenchymal stem cells: an overview of their potential in cellbased therapy. Expert Opin Biol Ther. 2015; 15(8): 1-14. CrossRef
Mathen C, Sawant MG, Gupta R, et al. Evaluation of potential application of Wharton's jelly-derived human mesenchymal stromal cells and its conditioned media for dermal regeneration using rat wound healing model. Cells Tissues Organs. 2021; 210(1): 31-44. CrossRef
Musatova IB, Volina VV, Chub OV, et al. Effects of implantation of cryopreserved placental explants on the behavioral indices and morphological characteristics of the cerebral structures in senescent mice. Neurophysiology. 2017; 49(5):363-71. CrossRef
Ozyazgan I, Liman N, Dursun N, Güneş I. The effects of ovariectomy on the mechanical properties of skin in rats. Maturitas. 2002; 43(1): 65-74. CrossRef
Prokopyuk VYu, Chub OV, Shevchenko NA, et al. Cryopreserved placental explants increase lifespan of male mice and change survival features of female mice. Probl Cryobiol Cryomed. 2017; 27(2): 143-50. CrossRef
Ranjbaran H, Abediankenari S, Mohammadi M, et al. Wharton's Jelly derived-mesenchymal stem cells: isolation and characterization. Acta Med Iran. 2018; 56(1): 28-33. PubMed
Roy A, Mantay M, Brannan C, Griffi ths S. Placental tissues as biomaterials in regenerative medicine. Biomed Res Int [Internet]. 2022 Apr 21 [Cited 11.11.2022]; 2022: 6751456. Available from: https://www.hindawi.com/journals/bmri/2022/6751456/ CrossRef
Thomas JR. Effects of age and diet on rat skin histology. Laryngoscope. 2005; 115(3): 405-11. CrossRef
Tsukahara K, Moriwaki S, Ohuchi A, et al. Ovariectomy accelerates photoaging of rat skin. Photochem Photobiol. 2001; 73(5): 525-31. CrossRef
Vasvani S, Kulkarni P, Rawtani D. Hyaluronic acid: A review on its biology, aspects of drug delivery, route of administrations and a special emphasis on its approved marketed products and recent clinical studies. Int J Biol Macromol. 2020; 151: 1012-29. CrossRef
Wong QYA, Chew FT. Defining skin aging and its risk factors: a systematic review and meta-analysis. Sci Rep [Internet]. 2021 Nov 11 [Cited 11.11.2022]; 11(1): 22075. Available from: https://www.nature.com/articles/s41598-021-01573-z CrossRef
Zaki SM. Characteristics of the skin of the female albino rats in different ages: histological, morphometric and electron microscopic study. J Cytol Histol [Internet]. 2015 Mar 11 [Cited 11.11.2022]; S3: 004. Available from: https://pdfs.semanticscholar.org/3481/dc9d515abcadbc7040b4fc3ff17c558b6558.pdf CrossRef
Zhang S, Duan E. Fighting against skin aging: the way from bench to bedside. Cell Transplant. 2018; 27(5): 729-38. CrossRef
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