Effect of Human Cord Blood Leukoconcentrate on Skin Regenerative Activity in Culture In Vitro

Authors

  • A. A. Tsutsayeva Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • V. V. Volina Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • L. V. Sokol Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • O. A. Zhulikov Kharkov Municipal Clinical Hospital N31, Kharkov

Keywords:

skin explants, human cord blood leukoconcentrate, in vitro culturing, morphofunctional activity

Abstract

The effect of human cord blood cryopreserved cell leukoconcentrate, suspended in autologous plasm (LHCB preparation) on in vitro cultured human skin morphology in different terms, has been studied. Skin was cultured on an agarised medium, supplemented with 17% embryonic serum (control) and 10% LHCB preparation (experimental group). The skin viability in vitro was maintained for 5–7 days longer in the presence of LHCB preparation compared to the control group. Under LHCB effect in cultured skin flaps there was an increase in fibroblast and fibrous structure amount, that enables concluding about the stimulation of both processes of fibroblast proliferation and synthesis of fibrous structures in derma.

Downloads

Published

2009-05-25

How to Cite

Tsutsayeva, A. A., Volina, V. V., Sokol, L. V., & Zhulikov, O. A. (2009). Effect of Human Cord Blood Leukoconcentrate on Skin Regenerative Activity in Culture In Vitro. Problems of Cryobiology and Cryomedicine, 19(1), 93–99. Retrieved from http://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/291

Issue

Vol. 19 No. 1 (2009): Problems of Cryobiology

Section

Cryomedicine, Clinical and Experimental Transplantology

License

Copyright (c) 2020 A. A. Tsutsayeva, V. V. Volina, L. V. Sokol, O. A. Zhulikov

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Authors who publish with this journal agree to the following terms:

  • Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  • Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  • Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).