Stimulating Effect of Low-Molecular Fraction from Cord Blood on Adhesion and Proliferation of Human Fibroblast Culture after Cryopreservation

Authors

  • A. K. Gulevsky Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • A. V. Trifonova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • T. F. Petrenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

cord blood fraction below 5 kDa, human fibroblasts, cryopreservation, adhesion, proliferation

Abstract

The effect of the fraction below 5 kDa, procured from cord blood, on adhesive and proliferative properties of human embryonic fibroblast diploid culture was studied. The mentioned fraction was shown as not affecting the efficiency of cell attachment, increasing the rate of cell flattening on substrate and stimulating proliferation of both native culture and after cryopreservation. There was concluded about a stimulating effect of cord blood fraction on human fibroblast culture growth, with demonstrating its reparative effect on a disordered cellular metabolism after cryopreservation.

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Published

2009-12-23

How to Cite

Gulevsky, A. K., Trifonova, A. V., & Petrenko, T. F. (2009). Stimulating Effect of Low-Molecular Fraction from Cord Blood on Adhesion and Proliferation of Human Fibroblast Culture after Cryopreservation. Problems of Cryobiology and Cryomedicine, 19(4), 395–405. Retrieved from http://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/228

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Section

Theoretical and Experimental Cryobiology