Lyophilized Human Cord Blood Leukoconcentrate to Treat Brain Ischemia in Rats

Authors

  • Volodymyr V. Lebedinets Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv; V.N. Karazin Kharkiv National University, Kharkiv
  • Ludmila V. Ostankova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Mykola O. Bondarovych Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv https://orcid.org/0000-0001-7977-0283
  • Olena D. Lutsenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv; Interdepartmental Scientific Center for Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Academy of Medical Sciences and Ministry of Health Care of Ukraine, Kharkiv https://orcid.org/0000-0003-3982-9687
  • Dmytro V. Lebedinets V.N. Karazin Kharkiv National University, Kharkiv
  • Igor G. Grisha Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv https://orcid.org/0000-0003-2875-910X
  • Maksym V. Ostankov Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv https://orcid.org/0000-0001-8412-5262
  • Kyrylo Yu. Parkhomenko Municipal Health Care Institution ‘Regional Clinical Hospital – Center for Emergency Care and Disaster Medicine’, Kharkiv
  • Anatoliy M. Goltsev Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv; Interdepartmental Scientific Center for Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Academy of Medical Sciences and Ministry of Health Care of Ukraine, Kharkiv https://orcid.org/0000-0002-5289-5876

DOI:

https://doi.org/10.15407/cryo32.01.044

Keywords:

lyophilization, cryopreservation, human cord blood leukoconcentrate, ischemic stroke, immune system, interferon-γ, interleukin-10, adhesive activity, phagocytic activity

Abstract

The paper outlines the experimental data on the impact of lyophilization on preservation of structural and functional characteristics of cells from human cord blood leukoconcentrate (HCBL). Lyophilization of HCBL was shown to ensure the preservation of cells with immune modulating potential in a heterogeneous population of HCBL. Lyophilized HCBL (lHCBL), to the same extent as cryopreserved one (cHCBL), revealed an equal immune correcting effect during ischemic brain injury in vivo in the experimental model of ischemic stroke (IS). The inclusion of lHCBL in therapy of brain ischemia demonstrated the improved indices of IFN-ү+ and IL-10+ spleen cells, adhesive and phagocytic activity of peritoneal cavity cells in rats with IS. The efficiency of using both lHCBL and cHCBL during IS may be associated with the implementation of therapeutic effect by presented among them cells and mediators, including multi-vector regulatory systems that maintain homeostatic stability of the body (immune, endocrine, nervous ones).

 

Probl Cryobiol Cryomed 2022; 32(1): 044–057

References

Dziedzic T. Systemic inflammation as a therapeutic target in acute ischemic stroke. Expert Rev Neurother. 2015; 15(5): 523-31. CrossRef

Goltsev AM, Mosiychuk VV, Goltsev KA, et al. inventors; Institute for Problems of Cryobiology and Cryomedicine, assignee. [Method of lyophilization of leukoconcentrate of cord blood]. Patent of Ukraine 117780U, 2017 July 10. Ukrainian.

Goltsev AM, Sokil LV, Stetsishin VG, et al. inventors; Institute for Problems of Cryobiology and Cryomedicine, assignee. [Method of lyophilization of leukoconcentrate of cord blood]. Patent of Ukraine 125846, 2018 May 25. Ukrainian.

Goltsev AM, Taranik GC, Grisha IG, et al. inventors; Institute for Problems of Cryobiology and Cryomedicine, assignee. [Method of lyophilization of leukoconcentrate of cord blood]. Patent of Ukraine 113006, 2017 Jan 10. Ukranian.

Goltsev AN, Lutsenko ED, Ostankov MV. [Effect of different cryopreservation regimens on manifestation of immune modulating activity of placenta at development of adjuvant artritis]. Problеms of cryobiology. 2008; 18(4): 456-8. Russian. Full Text

Goltsev AN, Stetsishin VG, Ostankova LV, et al. [The effect of lyophilized cord blood on the structure of the uterus of rats with herpesvirus infection.] In: [Proceedings of the second All-Ukrainian scientific-practical conference with international participation «Theory and practice of modern morphology»]; 2018 June 10-12 Dnipro, Ukraine. Dnipro; 2018. p. 159-60. Ukrainian.

Goltsev AN, Volina VV, Ostankov MV, et al. Effect of cryopreservation on functional properties of human cord blood leukoconcentrate nucleated cells. Problеms of cryobiology. 2010; 20 (1): 66-72. Full Text

Goltsev KA, Parkhomenko KYu, Krivoruchko IA, et al. [Treatment of peritonitis with lyophilized cord blood (experimental study)] In: [Proceedings of the scientific-practical conference with international participation, dedicated to the 20th anniversary of the founding of the Day of the Pharmaceutical Worker of Ukraine. Modern pharmacy: history, realities and prospects of development]; 2019 September 19-20 Kharkiv, Ukraine. Kharkiv; 2019. p. 286-8.

Goltsev KA, Ostankov MV, Kozhina OYu, et al. [Application of cryopreserved cord blood in combined therapy of acute purulent peritonitis.] Scientific Bulletins of the Belgorod State University. 2013; (18): 114-9. Russian.

Gontschar IA, Stepanova JI, Prudyvus IS. [Biochemical predictors and markers of ischemic stroke]. Minsk: Belarusian Medical Academy of Postgraduate Education; 2013. 512 p. Russian.

Grischenko VI, Goltsev AN. Transplantation of the products of embryofetoplacental complex. From understanding of mechanism of the effect to increasing the efficiency of application. Problеms of Сryobiology. 2002; (1): 54-85.

Javidi E, Magnus T. Autoimmunity after ischemic stroke and brain injury. Front Immunol. [Internet]. 2019 Apr 2 [cited 2020 June 15]; 10:686. Available from: https://www. frontiersin.org/articles/10.3389/fi mmu.2019.00686/full CrossRef

Jorgovanovic D, Song M, Wang L, et al. Roles of IFN-γ in tumor progression and regression: a review. Biomark Res. [Internet]. 2020 Sep 29 [cited 2021 June 15]; 8:49. Available from: https://biomarkerres.biomedcentral.com/articles/10.1186/s40364-020-00228-x CrossRef

Koval AK, Ostankov MV, Bondarovich NA, et al. Effect of cryopreserved cord blood on functional state of dendritic cells of rat skin with atopic dermatitis. Probl.Cryobiol Cryomed. 2018; 28(2): 159. CrossRef

Lebedinets VV, Ostankova LV, Dubrava TG, et al. [Use of cryopreserved cord blood to correct immune system in a model of ischemic stroke]. Medytsyna Sogodni i Zavtra. 2015; 69(4): 20-9. Russian.

Menshikov VV. [Laboratory methods for research in clinics]. Moscow: Meditsina, 1987. P. 123-5. Russian.

Merkulov GA. [The course in pathological histology technique]. Leningrad: Medgiz; 1969. 423 p. Russian.

Mishchenko TS. [Epidemiology of neurological diseases in Ukraine]. Neyro-News. 2008; (3): 107. Ukranian.

Paster EU, Gadfly BB, Pozur VK, et al. [Immunology laboratory course]. Кyiv: Vyshcha shkola; 1989. 304 p. Russian.

Petrovic-Djergovic D, Goonewardena SN, Pinsky DJ. Inflammatory disequilibrium in stroke. Circ Res. 2016; 119(1): 142-58. CrossRef

Sanberg PR, Ehrhart J. A hallmark clinical study of cord blood therapy in adults with ischemic stroke. Cell Transplant. 2019; 28(9-10): 1329-32. CrossRef

Serebrennikova SN, Seminskу IZh, Semenov NV, et al. [Interleukin-1, interleukin-10 in regulation of inflammatory process]. Siberian Medical Journal. 2012; (8): 5-7. Russian.

Shereshkov SI [Culturing of hemopoietic cells on semisolid nutrient media]. Laboratornoe delo. 1974; (3): 146-50. Russian.

Shearer WT, Lubin BH, Cairo MS, et al. Cord blood banking for potential future transplantation. [Internet] Pediatrics. 2017 Nov [cited 2020 June 15]; 140(5): e20172695. Available from: https://pediatrics.aappublications.org/content/140/5/e20172695.long CrossRef

Shipulin VM, Chumakova SP, Pogonchenkova DA, et al. [Interleukin-10 and non-classical monocytes as biomarkers of ischemic cardiomyopathy]. Patologiya krovoobrashcheniya i kardiokhirurgiya. 2020; 24(1): 45-53. Russian. CrossRef

Tsutsaieva AO, Hryschenko VI, Kudokotseva OV, et al., inventors; Institute for Problems of Cryobiology and Cryomedicine, assignee. [Method for cryopreservation of hemopoietic cells of cord blood]. Patent of Ukraine 31847А, 2000 December 15. Ukrainian.

Zapadnyuk IP, Zapadnyuk VI, Zakharia EA, et al. [Laboratory animals]. Kyiv: Vyscha shkola, 1983. 252 p. Russian.

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Published

2022-06-03

How to Cite

Lebedinets , V., Ostankova , L., Bondarovych , M., Lutsenko , O., Lebedinets , D., Grisha , I., Ostankov, M., Parkhomenko , K., & Goltsev, A. (2022). Lyophilized Human Cord Blood Leukoconcentrate to Treat Brain Ischemia in Rats . Problems of Cryobiology and Cryomedicine, 32(1), 44–57. https://doi.org/10.15407/cryo32.01.044

Issue

Section

Cryomedicine, Clinical and Experimental Transplantology