Cord Blood Cryopreservation with PEO-1500

Authors

  • V. V. Ryazantsev Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • L. A. Babijchuk Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • O. L. Zubova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • T. M. Gurina Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

cryopreservation, cord blood, erythrocytes, plasma

Abstract

The method without washing-out for the whole cord blood cryopreservation with PEO-1500, enabling to preserve up to 99 and 93% erythrocytes after freeze-thawing and by the transfusion test data, correspondingly, has been designed. The integrity of biologically active substances in plasm at the control level has been established. After cryopreservation with the mentioned above method the immunofluorescent test has demonstrated the integrity of up to 70% blood lymphocytes, including cord blood stem cells (CD34+).

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Published

2007-10-25

How to Cite

Ryazantsev, V. V., Babijchuk, L. A., Zubova, O. L., & Gurina, T. M. (2007). Cord Blood Cryopreservation with PEO-1500. Problems of Cryobiology and Cryomedicine, 17(3), 256–262. Retrieved from http://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/497

Issue

Section

Cryopreservation of Biological Resources