Effect of Cryopreservation Regimens on Behavior of SPEV Cells in Culture

Authors

  • Igor F. Kovalenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • Svetlana Ye. Kovalenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • Igor P. Vysekantsev Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • Tatyana F. Petrenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • Leonid F. Rozanov Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • Nadezhda A. Chernobay Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

DOI:

https://doi.org/10.15407/cryo26.02.116

Keywords:

SPEV cells, adhesion, cryopreservation

Abstract

This work demonstrates the ability of using the substrate-adhered SPEV cells as the object for studying the kinetics of osmotic reactions when exposing them to cryoprotective media. Permeability coefficients of SPEV cell membranes for the molecules of water and dimethyl sulfoxide (DMSO) have been determined. Using the physical-mathematical model for cell suspension freezing and calculated permeability coefficients the osmotic SPEV cell behavior during cryopreservation with different cooling rates has been simulated. The experiments on freezing the SPEV cell suspension showed that the cooling rate of 1.5 deg/min could ensure the optimal protection of the cells against damage in 1M DMSO presence. It has been demonstrated that the cooling rate above and below the optimum may cause a cell death as the result of intracellular crystallization or dehydration.

 

Probl Cryobiol Cryomed 2016; 26(2):116-123.

Author Biographies

Igor F. Kovalenko, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Department of Low Temperature Preservation

Svetlana Ye. Kovalenko, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Department of Low Temperature Preservation

Igor P. Vysekantsev, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Department of Cryomicrobiology

Tatyana F. Petrenko, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Department of Cryomicrobiology

Leonid F. Rozanov, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Department of Low Temperature Preservation

Nadezhda A. Chernobay, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Department of Low Temperature Preservation

References

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Gordienko E.A., Pushkar N.S. Physical grounds of low-temperature preservation for cell suspensions. Kiev: Naukova dumka; 1994.

Kovalenko I.F., Koschiy S.V., Timofeeva E.V. et al. Permeability of SPEV cell membranes for water and DMSO molecules. Problems of Cryobiology 2009; 19(1): 25–31.

Orlov A.I. Applied Statistics. Moscow: Ekzamen; 2006.

Schipitsyn M.A., Dmitrieva E.A., Voropaeva O.V. Investigation of influence of incubation medium composition and substrate on adhesion of erythroblastic islands. Modern High Technologies 2006; 3: 44.

Sergeev V.A., Sobko Y.A. Cell culture in veterinary and biotechnology. Kiev: Urozhay; 1990.

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Published

2016-06-23

How to Cite

Kovalenko, I. F., Kovalenko, S. Y., Vysekantsev, I. P., Petrenko, T. F., Rozanov, L. F., & Chernobay, N. A. (2016). Effect of Cryopreservation Regimens on Behavior of SPEV Cells in Culture. Problems of Cryobiology and Cryomedicine, 26(2), 116–123. https://doi.org/10.15407/cryo26.02.116

Issue

Vol. 26 No. 2 (2016): Probl Cryobiol Cryomed

Section

Theoretical and Experimental Cryobiology

License

Copyright (c) 2020 Igor F. Kovalenko, Svetlana Ye. Kovalenko, Igor P. Vysekantsev, Tatyana F. Petrenko, Leonid F. Rozanov, Nadezhda A. Chernobay

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