The Effect of DMSO of Different Concentrations and Freeze-Thawing on Newborn Rat Neural Cell Survival
Keywords:newborn rats, neural cells, freeze-thawing, viability, culturing
The effect of cryoprotectant dimethyl sulfoxide of different concentrations on the ability of isolated neural cells of newborn rats to proliferate and to be stained with trypan blue before and after freeze-thawing was studied. The cryoprotectant concentrations of 7.5 and 10% were established as the optimal ones, which preserved the ability of the neural stem/progenitor cells of newborn rats to proliferate and differentiate after freeze-thawing. Preservation of the functional activity of newborn rat neural cells demanded the presence of serum in freezing medium. It was demonstrated that the viability of newborn rat neural cells, assessed by trypan blue exclusion, only partially reflected the cell functional activity.
How to Cite
Copyright (c) 2020 T. D. Lyashenko, A. N. Sukach
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).