The Effect of DMSO of Different Concentrations and Freeze-Thawing on Newborn Rat Neural Cell Survival

Authors

  • T. D. Lyashenko G.S. Skovoroda Kharkov National Pedagogical University, Kharkov
  • A. N. Sukach G.S. Skovoroda Kharkov National Pedagogical University, Kharkov Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

newborn rats, neural cells, freeze-thawing, viability, culturing

Abstract

The effect of cryoprotectant dimethyl sulfoxide of different concentrations on the ability of isolated neural cells of newborn rats to proliferate and to be stained with trypan blue before and after freeze-thawing was studied. The cryoprotectant concentrations of 7.5 and 10% were established as the optimal ones, which preserved the ability of the neural stem/progenitor cells of newborn rats to proliferate and differentiate after freeze-thawing. Preservation of the functional activity of newborn rat neural cells demanded the presence of serum in freezing medium. It was demonstrated that the viability of newborn rat neural cells, assessed by trypan blue exclusion, only partially reflected the cell functional activity.

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Published

2011-09-19

How to Cite

Lyashenko, T. D., & Sukach, A. N. (2011). The Effect of DMSO of Different Concentrations and Freeze-Thawing on Newborn Rat Neural Cell Survival. Problems of Cryobiology and Cryomedicine, 21(3), 263–273. Retrieved from https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/124

Issue

Vol. 21 No. 3 (2011): Problems of Cryobiology

Section

Theoretical and Experimental Cryobiology

License

Copyright (c) 2020 T. D. Lyashenko, A. N. Sukach

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