Characterization of Immunophenotype and Differentiation Potential of Human Bone Marrow Mesenchymal Stromal Cells after Cryopreservation
Keywords:cryopreservation, mesenchymal stromal cells, human bone marrow, adipogenic and osteogenic differentiation, immunophenotype
Stromal cells derived from adult human bone marrow (BM) after in vitro expansion demonstrate a specific immunophenotype and ability to multilineage differentiation, inherent to mecenchymal stromal cells (MSCs)m. It was established that cryopreservation of human BM MSCs by two-step freezing under protection of 10% DMSO enables to preserve their immunophenotype and ability to induced osteogenic and adipogenic differentiation in vitro.
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Copyright (c) 2020 Yu. A. Petrenko, N. G. Skorobogatova, N. A. Volkova, A. Yu. Petrenko
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