Characterization of Immunophenotype and Differentiation Potential of Human Bone Marrow Mesenchymal Stromal Cells after Cryopreservation

Authors

  • Yu. A. Petrenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • N. G. Skorobogatova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • N. A. Volkova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • A. Yu. Petrenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

cryopreservation, mesenchymal stromal cells, human bone marrow, adipogenic and osteogenic differentiation, immunophenotype

Abstract

Stromal cells derived from adult human bone marrow (BM) after in vitro expansion demonstrate a specific immunophenotype and ability to multilineage differentiation, inherent to mecenchymal stromal cells (MSCs)m. It was established that cryopreservation of human BM MSCs by two-step freezing under protection of 10% DMSO enables to preserve their immunophenotype and ability to induced osteogenic and adipogenic differentiation in vitro.

Downloads

Published

2011-01-10

How to Cite

Petrenko, Y. A., Skorobogatova, N. G., Volkova, N. A., & Petrenko, A. Y. (2011). Characterization of Immunophenotype and Differentiation Potential of Human Bone Marrow Mesenchymal Stromal Cells after Cryopreservation. Problems of Cryobiology and Cryomedicine, 20(4), 436–442. Retrieved from https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/172

Issue

Vol. 20 No. 4 (2010): Problems of Cryobiology

Section

Cryopreservation of Biological Resources

License

Copyright (c) 2020 Yu. A. Petrenko, N. G. Skorobogatova, N. A. Volkova, A. Yu. Petrenko

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Authors who publish with this journal agree to the following terms:

  • Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  • Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  • Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).