Correction of Cytokine Profile in Autoimmune Diseases with Embryofetoplacental Complex Cryopreserved Products
Keywords:adjuvant arthritis, cytokines, lyophilization, human cord blood leukoconcentrate, placental cryoextract
In this work, we experimentally substantiated the possibility of correcting the cytokine profile to reduce the inflammation in mice with adjuvant arthritis (AA) via combined use of lyophilized human cord blood leukoconcentrate (lHCBL) and cryopreserved human placental extract (cHPE). Adjuvant arthritis was modelled in CBA/H mice. To day 7 after pathology induction, the animals were injected with lHCBL and cHPE singly or in combination. To days 14 and 28 of observation, all the introduced products showed a therapeutic effect. To 28 day of observation, the efficiency of combined use of lHCBL and cHPE was 3.1 and 2.5 times higher vs. the monotherapy (respectively), likely resulting from their synergy in respect to a decrease in arthritis index, TNFα concentration, impact of lHCBL and cHPE on IFNγ and IL-6 levels, correspondingly.
Probl Cryobiol Cryomed 2022; 32(2):121–133
Alnabhan R, Madrigal A, Saudemont A. Differential activation of cord blood and peripheral blood natural killer cells by cytokines. Cytotherapy. 2015; 17(1): 73-85. CrossRef
Balabanova RM, Vratskih EV, Ospelnikova TP, Kiseleva V.I. [Interferon system in patients with rheumatoid arthritis infected with hepatitis В and С viruses]. Rheumatology Science and Practice. 2003; 41 (4): 15-8. Russian. CrossRef
Chereshnev VA, Gusev EYu. [Immunology of inflammation: the role of cytokines]. Medical immunology. 2001; 3(3): 361-8. Russian.
Goltsev AN, Dubrava TG, Lutsenko ED, et al. A search for the methods of modification of the immune reactivity of the allomyelotransplant, which are alternative to cryopreservation. Part I. Problems of Cryobiology. 1996; (4): 3-16.
Goltsev AM, Fuller BJ, Bondarovych MO, et al. COVID-19 as a potential target for cryobiology and cryomedicine. Probl Cryobiol Cryomed 2020; 30(2):107-31. CrossRef
Goltsev AM, Ostankova LV, Lutsenko OD, et al, inventors. Institute for Problems of Cryobiology and Cryomedicine of NAS of Ukraine, assignee. [A method of comparative assessment of the effectiveness of treatment]. Patent of Ukraine 3761. 2004 Dec 15. Ukrainian.
Grischenko NG, Grischenko VI, Smolyaninova EI, et al. Effect of placental cryoextract on superovulation induction in mice with chronic ovary inflammation. Problems of Cryobiology. 2010; 20(3): 327-37. Full Text
Gromova OA, Torshin IY, Dibrova EA, et al. [Human placenta hydrolyzates in reconstructive therapy of connective tissue of cartilage and joints]. Meditsinskiy sovet. 2017; (5):178-85. Russian. CrossRef
Gulevskiy AK, Gorina OL, Moyseeva NN. [Influence of low-molecular fraction of cord blood on phagocytic activity of leukocytes, subjected to hypothermic storage]. World of Medicine and Biology. 2011; (1): 9-14. Russian.
Gulida МО, Miroshnichenko EV, Berezka МI, et al. [Application of placenta extract in complex treatment of patients with rheumatoid arthritis]. Experimental and clinical medicine. 2014; 62(1): 168-71. Russian.
Horwitz ME, Chao NJ, Rizzieri DA, et al. Umbilical cord blood expansion with nicotinamide provides long-term multilineage engraftment. J Clin Invest. 2014; 124(7): 3121-8. CrossRef
Koval GK, Lutsenko OD, Grisha IG, et al. Impact of lyophilisation on integrity of structural and functional characteristics of human cord blood leukoncentrate. Probl Cryobiol Cryomed. 2019; 29(4): 332-43. CrossRef
Kozhina OYu, Ostankov MV, Ostankova LV, et al. Effect of cryopreserved cord blood on activity of alveolar macrophages in experimental model of influenza. Probl Cryobiol Cryomed. 2013; 23(3): 247-59. Full Text
Mantovani G, Macciò A, Astara G, et al. Membrane-bound and soluble IL-2 receptors (p55 and p75 chains) on peripheral blood mononuclear cells from patients with solid malignancies. Cell Biophysic. 1993; 22: 79-99. CrossRef
McNiece IK, Bertoncello I, Kriegler AB, Quesenberry PJ. Colony-forming cells with high proliferative potential (HPP-CFC). Int J Cell Cloning. 1990; 8(3): 146-60. CrossRef
Menshikov VV, Deletorskay IH, Zolotnitskay RP. [Laboratory methods of research in clinic.]. Мoscow: Meditsina; 1987. 368 p. Russian.
Mischenko OY, Kotvitska AA. [Pharmacological efficacy of the analbic emulsion on the model of adjuvant arthritis in rats]. Visnyk farmatsii. 2001; (3): 124-5. Ukrainian.
Nasonov EL. [Pharmacotherapy for rheumatoid arthritis: new strategy, new targets]. Rheumatology Science and Practice. 2017; 55(4): 409-19. Russian. CrossRef
Ovchinnikova OV, Bondarenko IA, Lazurenko VV, et al. Application of cryopreserved cord blood in treatment of hypoferric anemia of pregnants. Problems of Cryobiology. 2008; 18 (3): 361-3. Full Text
Papait A, Vertua E, Magatti M, et al. Mesenchymal stromal cells from fetal and maternal placenta possess key similarities and differences: potential implications for their applications in regenerative medicine. Cells [Internet]. 2020 Jan 6 [cited 2021 Jul 13]; 9(1): 127. Available from https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7017205/ CrossRef
Prokopyuk OS, Chizhevsky VV, Prokopyuk VYu, et al. [Verification of the biosafety of cryopreserved placental objects]. In: Grischenko VI, Yurchenko TN, editors. [Placenta: cryopreservation, structure, properties and perspectives of clinical application]. Kharkiv; 2011. P. 207-44. Russian.
Struchkov PV, Konstantynova NA, Lavrentev VV, Chuchalyn AH. [Screening test for the evaluation of pathogenic properties of circulating immune complexes]. Laboratornoye delo. 1985; (7): 410-2. Russian.
How to Cite
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).