Ð¡ryopreservation Effect on Proliferation Characteristics and Differentiation Potential of Cultured Chorion Cells
Keywords:cryopreservation, chorion cell culture, viability, proliferation, differentiation
Five programs of chorion cell culture (ChCC) cryopreservation which differ by the cooling rate and duration have been studied in the work. The properties such as viability, adhesion, proliferation and directed differentiation were studied using culturing, light and fluorescence microscopy. The program comprising the cooling down to â€“6Â°C with the rate of 1Â°C/min, seeding with following cooling down to â€“80Â°C with the rate of 10Â°C/min provides both high integrity of ChCC population and survival of the fraction of multipotent cells.
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Copyright (c) 2020 E. I. Goncharuk, N. A. Volkova, V. I. Grischenko
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