Сryopreservation Effect on Proliferation Characteristics and Differentiation Potential of Cultured Chorion Cells

Authors

  • E. I. Goncharuk Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • N. A. Volkova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • V. I. Grischenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

cryopreservation, chorion cell culture, viability, proliferation, differentiation

Abstract

Five programs of chorion cell culture (ChCC) cryopreservation which differ by the cooling rate and duration have been studied in the work. The properties such as viability, adhesion, proliferation and directed differentiation were studied using culturing, light and fluorescence microscopy. The program comprising the cooling down to –6°C with the rate of 1°C/min, seeding with following cooling down to –80°C with the rate of 10°C/min provides both high integrity of ChCC population and survival of the fraction of multipotent cells.

Downloads

Published

2010-09-20

How to Cite

Goncharuk, E. I., Volkova, N. A., & Grischenko, V. I. (2010). Сryopreservation Effect on Proliferation Characteristics and Differentiation Potential of Cultured Chorion Cells. Problems of Cryobiology and Cryomedicine, 20(3), 309–317. Retrieved from https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/191

Issue

Vol. 20 No. 3 (2010): Problems of Cryobiology

Section

Cryopreservation of Biological Resources

License

Copyright (c) 2020 E. I. Goncharuk, N. A. Volkova, V. I. Grischenko

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Authors who publish with this journal agree to the following terms:

  • Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  • Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  • Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).