Cryopreservation of Pleurotus Mycelium Pleurotus ostreatus of Different Strains According to Three Freezing Programs

Authors

  • L. G. Chernyshenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • L. E. Shatilova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • L. V. Stepanyuk Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

cryopreservation, mycelium, Pleurotus ostreatus

Abstract

Morphofunctional properties of pleurotus mycelium Pleurotus ostreatus of different strains after cryopreservation according to three freezing programs were investigated. The cryopreservation program 1 was found as optimal cooling at the rate of 1–4°C/min down to -28°C/min, 20 min stabilization, further cooling down to -100°C at a rate of 30°C/min, fast submerging into liquid nitrogen. The viability of cryopreserved mycelium on different programs was established as not changed.

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Published

2008-12-22

How to Cite

Chernyshenko, L. G., Shatilova, L. E., & Stepanyuk, L. V. (2008). Cryopreservation of Pleurotus Mycelium Pleurotus ostreatus of Different Strains According to Three Freezing Programs. Problems of Cryobiology and Cryomedicine, 18(4), 413–416. Retrieved from https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/341

Issue

Vol. 18 No. 4 (2008): Problems of Cryobiology

Section

Conference Papers

License

Copyright (c) 2020 L. G. Chernyshenko, L. E. Shatilova, L. V. Stepanyuk

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