Study of the Effect of Cryoprotectants on Liposomes of Canine Spermatozoa Total Lipids

Authors

  • T. P. Linnik Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • T. S. Dyubko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • M. I. Yegorov Kiev National University named by Taras Shevchenko
  • V. G. Pivovarenko Kiev National University named by Taras Shevchenko

Keywords:

dog, spermatozoa, lipids, cryoprotectants, fluorescent probe, FME

Abstract

The mechanism of interaction between the cryoprotective agents (CPAs): ethylene glycol (EG), glycerol and N,N-dimethylformamide (DMFA) with lipid bilayer of liposomes formed from total lipids of canine spermatozoa by fluorescent spectroscopy using new fluorescent probe of flavonoid origin: 3-hydroxy-4’-(N,N-dimethylamine)flavon (FME). It has been established that among the studied CPAs (glycerol, EG, DMFA) the highest effect on the form of FME fluorescence spectrum associated to lipid bilayer is rendered by DMFA. The effect rate of the studies CPAs on liposomes is determined by hydrophobic-hydrophilic balance of their molecules. DMFA predominantly affects lipid bilayer by hydrophobic interaction, and glycerol and EG mainly influence hydrate layer of polar “heads†of phospholipids by forming intermolecular hydrogen bonds.

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Published

2007-06-15

How to Cite

Linnik, T. P., Dyubko, T. S., Yegorov, M. I., & Pivovarenko, V. G. (2007). Study of the Effect of Cryoprotectants on Liposomes of Canine Spermatozoa Total Lipids. Problems of Cryobiology and Cryomedicine, 17(2), 141–146. Retrieved from https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/513

Issue

Vol. 17 No. 2 (2007): Problems of Cryobiology

Section

Theoretical and Experimental Cryobiology

License

Copyright (c) 2020 T. P. Linnik, T. S. Dyubko, M. I. Yegorov, V. G. Pivovarenko

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