Application of Plastic Strain Method to Determine Threshold Concentrations for Cryoprotective Substances During Saccharomyces cerevisiae Yeast Cell Cryopreservation

Authors

  • A. I. Osetsky Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • A. L. Kirilyuk Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • T. M. Gurina Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • I. P. Vysekantsev Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

plastic strain, fluidity threshold, stress-strain curves, glycerol, DMSO, PEO-1500, Saccharomyces cerevisiae

Abstract

Plastic strain of glycerol and PEO-1500 frozen aqueous solutions has been studied and an alteration in their plastic characteristics when achieving certain (threshold) concentrations of cryoprotective substances has been established. This change is associated to local closed liquid phase inclusion transition into the through liquid interlayers, that excludes an internal pressure development and corresponding mechanical damages in biological objects being under cryopreservation. This conclusion is confirmed by the experiments on Saccharomyces cerevisiae yeast cell cryopreservation.

Downloads

Published

2007-03-15

How to Cite

Osetsky, A. I., Kirilyuk, A. L., Gurina, T. M., & Vysekantsev, I. P. (2007). Application of Plastic Strain Method to Determine Threshold Concentrations for Cryoprotective Substances During Saccharomyces cerevisiae Yeast Cell Cryopreservation. Problems of Cryobiology and Cryomedicine, 17(1), 71–79. Retrieved from https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/534

Issue

Vol. 17 No. 1 (2007): Problems of Cryobiology

Section

Theoretical and Experimental Cryobiology

License

Copyright (c) 2020 A. I. Osetsky, A. L. Kirilyuk, T. M. Gurina, I. P. Vysekantsev

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Authors who publish with this journal agree to the following terms:

  • Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  • Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  • Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).