Extracts of Cryopreserved Fragments of Pig Spleen and Piglet Skin Affect the Healing of Cold Wounds in Rats
DOI:
https://doi.org/10.15407/cryo25.02.151Keywords:
cold trauma, healing, extract, skin, spleenAbstract
The optimizing of cold wound treatment has been currently remained a relevant task, which can be particularly solved through the introduction of the combined therapy with immune biological products. The experimental animals were injected with 1 ml extract of cryopreserved fragments of either newborn piglet skin or pig spleen into abdominal cavity once a day within the course of experiment. The dose of peptides was 50 µg per 100 g of an animal. The control rats were injected with 1 ml of physiological saline once a day. It has been established that introduction to the animals with cold trauma of the extract of cryopreserved fragments of either newborn piglet skin or pig spleen accelerates the healing of cold wounds. Starting from day 7 the area of wounds in the animals, which were injected with the extract of cryopreserved fragments of either newborn piglet skin or pig spleen was statistically and significantly lower than in the control rats. In addition, in the experimental animals the index of blood leukocytes shift was more rapidly normalized. After introduction of the extracts to the animals with cold trauma the rate of free radical oxidation of lipids and the TBARS level in blood serum of the animals with cold injury were reduced. Thus, to day 14 of the experiment in the groups with introduced extract of cryopreserved fragments of either pig spleen or newborn piglet skin the level of TBARS was normalized, and in the control group this happened only to day 21. In the animals introduced with the extracts, the ligand-loading of albumin decreased faster than in the control animals.
Probl Cryobiol Cryomed 2015; 25(2): 151-161.
References
Arefyeva T.I., Sokolov V.O., Pylaeva E.A. et al. Peptide fragment of 29-40 of amino acid sequence of monocytic chemotactic protein-1 (MPC-1) stimulates the migration of monocytes in vivo and provides wounds healing. Doklady Akademii Nauk 2012; 446 (1): 106–109.
Arford S. Treatment of frostbite: a cold-induced injury. J Wound Ostomy Continence Nurs 2008; 35 (6): 625–630. CrossRef PubMed
Arutyunyan A.V., Dubinina E.E., Zybina N.N. Methods of asses-sing of free radical oxidation and antioxidant system of a body. Metod. Recommendations. St. Petersburg: Foliant; 2000.
Askarov T.A., Memetov F.U., Batyrbekov A.A. et al. Influence of Lacto Flor at immune status of animals with burn disease and hormone-induced immune deficiency. Problemy Biologii i Meditsiny 2003; 1–3: 14-17.
Babenko G.A., Gonskiy Ya.I., Antonik I.M. et al. About role of metals in the process of free radical oxidation in organism's tissues on data of spontaneous and induced chemilumi-nescence. In: Chemiluminescence. Moscow: Nauka; 1983. p. 164–178.
Babskaya Y.E., Lavrov V.A., Omonina N.A. Intensity of free radical oxidation of lipids in acute period of burn disease. Khirurgiya 1985; 11: 95–97.
Bespalova I., Belochkina I., Galchenko S., Sandomirsky B. Orga-nospecific influence of the extract of cryopreserved piglet's skin fragments. Biological effect of cNPSE and cPSE in fibro-blast culture. Periodicum Biologorum 2014; 116 (1): 99–103.
Byzov V.V., Vysekantsev I.P., Galchenko S.Ye. et al. Effect of endobronchial introduction of extracts of cryopreserved xeno-spleen fragments on some local immunity factors in complex therapy of patients with lungs abscesses. Problems of Cryobiology 2001; 4: 65–70.
Chadaev A.P., Sviridov S.V., Klimiashvili A.D. et al. Cold injury. Ros Med Zhurnal 2005; 35: 20–23.
Chorna I.O., Ligonenko O.V., Girin L.V. Changes at the system of homeostasis under the influence of peptidic bioregulators in the time of injury treatment. Klin Khirurgiya 2002; (11–12): 77–78.
Dontsov V.I. Regulation of cell growth of somatic tissues by lymphocytes and new theory of aging. Review. Profilaktika Stareniya 1998; 1: 40–63.
Dyubko T.S., Sidorov V.I., Sokolik O.A. et al. Comparative analysis of fluorescent stain K-35 and K7-1045 interaction with proteins of rat's blood serum. Journal of V.N. Karazin Kharkiv National University. Series: biology. 2009; 9 (856): 11–18.
Eming S.A., Whitsitt J.S., He L. Particle-mediated gene transfer of PDGF isoforms promotes wound repair. J Invest Dermatol 1999; 112 (3): 297–302. CrossRef PubMed
Galchenko S.Ye. Extracts of cryopreserved fragments of xenoorgans: obtaining and biological effect. Problems of Cryobiology 2005; 15 (3): 403–406.
Gryzunov U.A. and Dobretsov G.E., editors. Blood serum albumin in clinical medicine. Book 2. Moscow: GEOTAR; 1998.
Ivanova S.V., Kirpichonok L.N. Application of fluorescent me-thods in medicine. Med Novosti 2008; 12: 56-61.
Kinoshita M., Seki S., Ono S. Paradoxical effect of IL-18 therapy on the severe and mild Escherichia coli infections in burn-injured mice. Ann Surg 2004; 240 (2): 313–320. CrossRef PubMed
Kozinets G.P., Slesarenko S.V., Radzihovsky A.P. et al. Burn intoxication. Pathogenesis, clinical picture, principle of treatment. Moscow: MEDpress-inform. 2005.
Lopukhin Yu.M., Vladimirov Yu.A., Molodenkov M.N. et al. Chemiluminescence registration of blood serum components in bivalent iron presence. Bul Experim Biologii i Meditsiny 1983; (2): 61–63.
Osikov M.V., Likhacheva A.G., Telesheva L.F. Inherent immunity rates and injury focus morphology at experimental thermal injury. Med Nauki Fundamentalnye Issledovaniya 2012; 8: 381–386.
Ostrovskiy V.K., Maschenko A.V., Yangolenko D.V. et al. Blood and leukocytic intoxication rates when assessing the severity and estimating the prognosis at inflammatory, purulent and pyo-destructive diseases. Klin Lab Diagnostika 2006; 6: 50–53.
Petrovich Yu.N., Gutkin D.V. Free radical oxidation and its role in inflammation, ischemia and stress pathogenesis. Patolog Fiziologia 1986; 5: 85–92.
Shestakov V.A., Bojchevskaya N.O., Sherstnyov M.P. Chemiluminescence of blood plasma in presence of hydrogen peroxide. Vopr Med Khimii 1979; 2: 132–137.
Shkodovskaya N.Yu., Gojdenko N.I., Galchenko S.Ye., Sandomirsky B.P. Experimental skin cold injuries in therapy by placenta extracts, officinal one and newborn piglets' skin. Problemy Med Nauky ta Osvity 2005; (4): 41–45.
Slesarenko S.V., Badul P.A. To the question for necessity of standardizing of medical aid at frostbite. Khirurgiya Ukrainy 2007; 4: 6–10.
Speranskiy I.I., Samoilenko G.E., Lobachova M.V. General blood analysis – are all its facilities exhausted? Integral indices of intoxication as criterion of assessing the severity of endogenous intoxication, its complication and efficiency of treatment implemented. Zdorovie Ukrainy 2009; 19(6): 51–57.
Yavorskaya V.A., Belous A.M., Mohamed A.N. Study of middle mass molecules level and processes of lipid peroxidation in the blood of patients with different forms of stroke. Zhurnal Nevrologii i Psikhiatrii 2000; 1: 48–51.
Yemelyanov A.Yu., Lipatov K.V., Farhat F.A. Frostibites: actual questions of pathogenesis, diagnosis and treatment. Khirurgiya 2002; 12: 59–63.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2020 Iryna G. Bespalova, Liliya A. Rogoza, Sergij Ye. Galchenko, Borys P. Sandomyrsky
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
