Extracts of Cryopreserved Fragments of Pig Spleen and Piglet Skin Affect the Healing of Cold Wounds in Rats

Authors

  • Iryna G. Bespalova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov, Ukraine
  • Liliya A. Rogoza Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov, Ukraine
  • Sergij Ye. Galchenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov, Ukraine
  • Borys P. Sandomyrsky Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov, Ukraine

DOI:

https://doi.org/10.15407/cryo25.02.151

Keywords:

cold trauma, healing, extract, skin, spleen

Abstract

The optimizing of cold wound treatment has been currently remained a relevant task, which can be particularly solved through the introduction of the combined therapy with immune biological products. The experimental animals were injected with 1 ml extract of cryopreserved fragments of either newborn piglet skin or pig spleen into abdominal cavity once a day within the course of experiment. The dose of peptides was 50 µg per 100 g of an animal. The control rats were injected with 1 ml of physiological saline once a day. It has been established that introduction to the animals with cold trauma of the extract of cryopreserved fragments of either newborn piglet skin or pig spleen accelerates the healing of cold wounds. Starting from day 7 the area of wounds in the animals, which were injected with the extract of cryopreserved fragments of either newborn piglet skin or pig spleen was statistically and significantly lower than in the control rats. In addition, in the experimental animals the index of blood leukocytes shift was more rapidly normalized. After introduction of the extracts to the animals with cold trauma the rate of free radical oxidation of lipids and the TBARS level in blood serum of the animals with cold injury were reduced. Thus, to day 14 of the experiment in the groups with introduced extract of cryopreserved fragments of either pig spleen or newborn piglet skin the level of TBARS was normalized, and in the control group this happened only to day 21. In the animals introduced with the extracts, the ligand-loading of albumin decreased faster than in the control animals.


Probl Cryobiol Cryomed 2015; 25(2): 151-161.

Author Biographies

Iryna G. Bespalova, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov, Ukraine

Department of Experimental Cryomedicine

Liliya A. Rogoza, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov, Ukraine

Department of Experimental Cryomedicine

Sergij Ye. Galchenko, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov, Ukraine

Department of Experimental Cryomedicine

Borys P. Sandomyrsky, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov, Ukraine

Department of Experimental Cryomedicine

References

Arefyeva T.I., Sokolov V.O., Pylaeva E.A. et al. Peptide fragment of 29-40 of amino acid sequence of monocytic chemotactic protein-1 (MPC-1) stimulates the migration of monocytes in vivo and provides wounds healing. Doklady Akademii Nauk 2012; 446 (1): 106–109.

Arford S. Treatment of frostbite: a cold-induced injury. J Wound Ostomy Continence Nurs 2008; 35 (6): 625–630. CrossRef PubMed

Arutyunyan A.V., Dubinina E.E., Zybina N.N. Methods of asses-sing of free radical oxidation and antioxidant system of a body. Metod. Recommendations. St. Petersburg: Foliant; 2000.

Askarov T.A., Memetov F.U., Batyrbekov A.A. et al. Influence of Lacto Flor at immune status of animals with burn disease and hormone-induced immune deficiency. Problemy Biologii i Meditsiny 2003; 1–3: 14-17.

Babenko G.A., Gonskiy Ya.I., Antonik I.M. et al. About role of metals in the process of free radical oxidation in organism's tissues on data of spontaneous and induced chemilumi-nescence. In: Chemiluminescence. Moscow: Nauka; 1983. p. 164–178.

Babskaya Y.E., Lavrov V.A., Omonina N.A. Intensity of free radical oxidation of lipids in acute period of burn disease. Khirurgiya 1985; 11: 95–97.

Bespalova I., Belochkina I., Galchenko S., Sandomirsky B. Orga-nospecific influence of the extract of cryopreserved piglet's skin fragments. Biological effect of cNPSE and cPSE in fibro-blast culture. Periodicum Biologorum 2014; 116 (1): 99–103.

Byzov V.V., Vysekantsev I.P., Galchenko S.Ye. et al. Effect of endobronchial introduction of extracts of cryopreserved xeno-spleen fragments on some local immunity factors in complex therapy of patients with lungs abscesses. Problems of Cryobiology 2001; 4: 65–70.

Chadaev A.P., Sviridov S.V., Klimiashvili A.D. et al. Cold injury. Ros Med Zhurnal 2005; 35: 20–23.

Chorna I.O., Ligonenko O.V., Girin L.V. Changes at the system of homeostasis under the influence of peptidic bioregulators in the time of injury treatment. Klin Khirurgiya 2002; (11–12): 77–78.

Dontsov V.I. Regulation of cell growth of somatic tissues by lymphocytes and new theory of aging. Review. Profilaktika Stareniya 1998; 1: 40–63.

Dyubko T.S., Sidorov V.I., Sokolik O.A. et al. Comparative analysis of fluorescent stain K-35 and K7-1045 interaction with proteins of rat's blood serum. Journal of V.N. Karazin Kharkiv National University. Series: biology. 2009; 9 (856): 11–18.

Eming S.A., Whitsitt J.S., He L. Particle-mediated gene transfer of PDGF isoforms promotes wound repair. J Invest Dermatol 1999; 112 (3): 297–302. CrossRef PubMed

Galchenko S.Ye. Extracts of cryopreserved fragments of xenoorgans: obtaining and biological effect. Problems of Cryobiology 2005; 15 (3): 403–406.

Gryzunov U.A. and Dobretsov G.E., editors. Blood serum albumin in clinical medicine. Book 2. Moscow: GEOTAR; 1998.

Ivanova S.V., Kirpichonok L.N. Application of fluorescent me-thods in medicine. Med Novosti 2008; 12: 56-61.

Kinoshita M., Seki S., Ono S. Paradoxical effect of IL-18 therapy on the severe and mild Escherichia coli infections in burn-injured mice. Ann Surg 2004; 240 (2): 313–320. CrossRef PubMed

Kozinets G.P., Slesarenko S.V., Radzihovsky A.P. et al. Burn intoxication. Pathogenesis, clinical picture, principle of treatment. Moscow: MEDpress-inform. 2005.

Lopukhin Yu.M., Vladimirov Yu.A., Molodenkov M.N. et al. Chemiluminescence registration of blood serum components in bivalent iron presence. Bul Experim Biologii i Meditsiny 1983; (2): 61–63.

Osikov M.V., Likhacheva A.G., Telesheva L.F. Inherent immunity rates and injury focus morphology at experimental thermal injury. Med Nauki Fundamentalnye Issledovaniya 2012; 8: 381–386.

Ostrovskiy V.K., Maschenko A.V., Yangolenko D.V. et al. Blood and leukocytic intoxication rates when assessing the severity and estimating the prognosis at inflammatory, purulent and pyo-destructive diseases. Klin Lab Diagnostika 2006; 6: 50–53.

Petrovich Yu.N., Gutkin D.V. Free radical oxidation and its role in inflammation, ischemia and stress pathogenesis. Patolog Fiziologia 1986; 5: 85–92.

Shestakov V.A., Bojchevskaya N.O., Sherstnyov M.P. Chemiluminescence of blood plasma in presence of hydrogen peroxide. Vopr Med Khimii 1979; 2: 132–137.

Shkodovskaya N.Yu., Gojdenko N.I., Galchenko S.Ye., Sandomirsky B.P. Experimental skin cold injuries in therapy by placenta extracts, officinal one and newborn piglets' skin. Problemy Med Nauky ta Osvity 2005; (4): 41–45.

Slesarenko S.V., Badul P.A. To the question for necessity of standardizing of medical aid at frostbite. Khirurgiya Ukrainy 2007; 4: 6–10.

Speranskiy I.I., Samoilenko G.E., Lobachova M.V. General blood analysis – are all its facilities exhausted? Integral indices of intoxication as criterion of assessing the severity of endogenous intoxication, its complication and efficiency of treatment implemented. Zdorovie Ukrainy 2009; 19(6): 51–57.

Yavorskaya V.A., Belous A.M., Mohamed A.N. Study of middle mass molecules level and processes of lipid peroxidation in the blood of patients with different forms of stroke. Zhurnal Nevrologii i Psikhiatrii 2000; 1: 48–51.

Yemelyanov A.Yu., Lipatov K.V., Farhat F.A. Frostibites: actual questions of pathogenesis, diagnosis and treatment. Khirurgiya 2002; 12: 59–63.

Downloads

Published

2015-06-20

How to Cite

Bespalova, I. G., Rogoza, L. A., Galchenko, S. Y., & Sandomyrsky, B. P. (2015). Extracts of Cryopreserved Fragments of Pig Spleen and Piglet Skin Affect the Healing of Cold Wounds in Rats. Problems of Cryobiology and Cryomedicine, 25(2), 151–161. https://doi.org/10.15407/cryo25.02.151

Issue

Section

Cryomedicine, Clinical and Experimental Transplantology