Influence of Autoclaved Placenta Extracts on Phase Behavior of Cell Suspensions Below 0°C

Authors

  • Aleksandra V. Zinchenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • Elena N. Bobrova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • Miroslav I. Schetinsky Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • Yulianna S. Govorova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

phase transitions, differential scanning calorimeter, placenta extract, autoclaving, cell suspensions, SPEV, erythrocytes, Candida albicans.

Abstract

Phase transitions in human placental extracts during cooling at -196°C and the effect of fresh and autoclaved extracts on phase transitions in cell suspensions were studied. It has been found that after cooling with the rate of 3.3(3)deg/s in cell suspensions or suspending media the process of crystallization termination developed at the stage of heating with the rate of 8.3(3)x10-3 deg/s. After dilution of the suspension of Candida albicans and SPEV cells with autoclaved extract this process was not observed. It was found that the crystallization of eutectic mixtures was developed in frozen placental extracts unlike the mixture of extracts with erythrocytes and C. albicans cells. Probl Cryobiol Cryomed 2013; 23(1):40-48.

Author Biographies

Aleksandra V. Zinchenko, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Department of Cryobiophysics

Elena N. Bobrova, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Department of Cryobiophysics

Miroslav I. Schetinsky, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Department of Cryobiophysics

Yulianna S. Govorova, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Department of Cryobiophysics

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Published

2013-03-15

How to Cite

Zinchenko, A. V., Bobrova, E. N., Schetinsky, M. I., & Govorova, Y. S. (2013). Influence of Autoclaved Placenta Extracts on Phase Behavior of Cell Suspensions Below 0°C. Problems of Cryobiology and Cryomedicine, 22(1), 40–48. Retrieved from https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/8

Issue

Vol. 22 No. 1 (2012): Problems of Cryobiology

Section

Theoretical and Experimental Cryobiology

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