Comparative Study of Influence of Cryopreservation Regimens on Free and Immobilized Cells of Saccharomyces boulardii Probiotic

Authors

  • I. P. Vysekantsev Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • O. M. Babinets Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • V. F. Martsenyuk Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • T. M. Gurina Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

probiotics, cryopreservation, immobilization on enterosorbents

Abstract

The influence of cooling regimens and preserving media composition on Saccharomyces boulardii probiotic free cells and those immobilized on enterosorbents, was comparatively studied. The preservation of higher viability of free yeast cells was established after application of all the cryopreservation regimens. The viability of ‘carrier-cells’ complexes was much lower. Cooling with 1 degree/min rate provided the maximum viability for free and immobilized cells.

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Published

2012-03-14

How to Cite

Vysekantsev, I. P., Babinets, O. M., Martsenyuk, V. F., & Gurina, T. M. (2012). Comparative Study of Influence of Cryopreservation Regimens on Free and Immobilized Cells of Saccharomyces boulardii Probiotic. Problems of Cryobiology and Cryomedicine, 22(1), 21–29. Retrieved from https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/94

Issue

Section

Theoretical and Experimental Cryobiology