Effect of Disperse Silica on Bound Water in Frozen Cellular Suspensions

Authors

  • V. V. Turov
  • S. P. Gorbik
  • A. A. Chuiko

Abstract

Using the 1H NMR method under conditions of liquid phase freezing-out the effect of disperse silica on hydration rate of model cell suspensions in varying the concentration of cell mass within the range of 0-25% mass and that of SiO2 within the range of 0‑5% (w/w) have been studied. Disperse silica was found to cause a strong decrease in bound water concentration in the system. The maximum reduction in inter-phase energy of cell/water (γS) was equal to 130 J/g. In concentrated cell suspension the cells and particles of silica can form complex colloid systems with the value of γS in two times lower than that in the binary system.

 

Author Biographies

V. V. Turov

Institute of the Surface Chemistry of the National Academy of Sciences of Ukraine, Kiev

S. P. Gorbik

Institute of the Surface Chemistry of the National Academy of Sciences of Ukraine, Kiev

A. A. Chuiko

Institute of the Surface Chemistry of the National Academy of Sciences of Ukraine, Kiev

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Published

2002-09-23

How to Cite

Turov, V. V., Gorbik, S. P., & Chuiko, A. A. (2002). Effect of Disperse Silica on Bound Water in Frozen Cellular Suspensions. Problems of Cryobiology and Cryomedicine, (3), 16–23. Retrieved from https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/953

Issue

No. 3 (2002): Problems of Cryobiology

Section

Theoretical and Experimental Cryobiology

License

Copyright (c) 2020 V. V. Turov, S. P. Gorbik, A. A. Chuiko

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