Effect of Cryoprotective Media Components on Dog Spermatozoa Viability During

Authors

  • T. P. Linnik
  • M. I. Egorov
  • T. S. Dyubko
  • A. P. Belonozhko

Keywords:

spermatozoa, motility, cryopreservation, cryoprotectants, albumin, egg yolk

Abstract

There was studied the effect of such cryoprotectants (CP) as: glycerol (GL), ethylene glycol (EG), dimethyl sulfoxide (DMSO), N-N-dimethyl formamide (DMF), 1,2-propanediol (1,2-PD) in 1 M concentration on dog spermatozoa (DS) viability before and after freeze-thawing in 3 media, differing by composition. The first medium (TFY) comprised the yolk of chicken eggs, the second one (ТFA) consisted of egg albumin, the third one (ТF) was used without additives. It was established, that the highest viability of DS both before and after cryopreservation was observed under DMF protection in TFA medium. A possible mechanism of egg albumin effect on DS viability in combination with DMF is discussed.

Author Biographies

T. P. Linnik

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

M. I. Egorov

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

T. S. Dyubko

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

A. P. Belonozhko

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Downloads

Published

2005-12-25

How to Cite

Linnik, T. P., Egorov, M. I., Dyubko, T. S., & Belonozhko, A. P. (2005). Effect of Cryoprotective Media Components on Dog Spermatozoa Viability During. Problems of Cryobiology and Cryomedicine, 15(4), 645–656. Retrieved from https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/1214

Issue

Vol. 15 No. 4 (2005): Problems of Cryobiology

Section

Cryopreservation of Biological Resources

License

Authors who publish with this journal agree to the following terms:

  • Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  • Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  • Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).