Efficiency of Combined Cryopreservatives Containing Glycerol or 1,2-Propanediol During Freezing of Erythrocytes

Authors

  • V. V. Ramazanov Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

erythrocytes, combined cryopreservatives, H ion flow, osmotic hemolysis

Abstract

The osmotic properties of erythrocytes frozen-thawed in combined cryopreservatives, containing polymeric non-penetrating (dextran, polyethylene glycol) and penetrating (glycerol, 1,2-propane diol) cryoprotectants were studied. It was established that in erythrocytes frozen-thawed in the presence of polymers the increasing of H+ ion flow rate and osmotic fragility in the environment with 0.45–0.9% NaCl was observed. It is sufficient to add 5% glycerol or 1,2-propane diol (1,2-PD) to the medium to preserve osmotic properties of frozen-thawed cells. The obtained results enable to suggest that cryoprotective efficiency of combined cryopreservatives, containing non-penetrating and penetrating cryoprotectants is determined both by the contribution of cryoprotective components differing by action mechanism into the sum protection efficiency during freezing-thawing and by the weakening of posthypertonic stress in cells during freeze-thawing.

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Published

2011-07-11

How to Cite

Ramazanov, V. V. (2011). Efficiency of Combined Cryopreservatives Containing Glycerol or 1,2-Propanediol During Freezing of Erythrocytes. Problems of Cryobiology and Cryomedicine, 21(2), 125–136. Retrieved from https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/138

Issue

Vol. 21 No. 2 (2011): Problems of Cryobiology

Section

Theoretical and Experimental Cryobiology

License

Copyright (c) 2020 V. V. Ramazanov

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