Influence Of Low-Molecular Fraction (Up To 5 Kda) Of Human Cord Blood On Growth Properties Of Culture Of Mesenchymal Stromal Cells Subjected To Cryopreservation

Authors

  • Oleksandr K. Gulevsky Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Aleksey A. Lavrik Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Anna V. Trifonova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Vladimir G. Karpenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

DOI:

https://doi.org/10.15407/cryo28.02.131

Keywords:

cryopreservation, human cord blood fraction below 5 kDa, mesenchymal stromal cells, proliferation, colony formation, mitotic regimen, immunophenotype, differentiation potential

Abstract

The influence of cryopreservation on growth properties, immune phenotype and differentiation potential of mesenchymal stromal cells (MSCs) of human and rat was investigated, as well as the ability of  human cord blood fractions below 5 kD (hCBF) to affect these indices  in thawed cultures. It has been  established that introduction of the hCBF with the concentration of 256-400 µg/ml to rat MSCs (rMSCs) culture caused the normalization of the cells growth properties reduced after cryopreservation that was not observed in human MSCs (hMSCs) culture. It has been shown that neither cryopreservation nor hCBF change the immune phenotype and ability for multi-lineage differentiation of hMSCs and rMSCs. The efficiency  of low molecular weight fraction dependent on physiological characteristics of cells and its concentration in culture medium was concluded.

Probl Cryobiol Cryomed 2018; 28(2): 131–138

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Published

2018-07-02

How to Cite

Gulevsky, O. K., Lavrik, A. A., Trifonova, A. V., & Karpenko, V. G. (2018). Influence Of Low-Molecular Fraction (Up To 5 Kda) Of Human Cord Blood On Growth Properties Of Culture Of Mesenchymal Stromal Cells Subjected To Cryopreservation. Problems of Cryobiology and Cryomedicine, 28(2), 131–138. https://doi.org/10.15407/cryo28.02.131

Issue

Vol. 28 No. 2 (2018): Probl Cryobiol Cryomed

Section

Theoretical and Experimental Cryobiology

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