Cryosensitivity of Mesenchymal Stromal Cells Encapsulated in Alginate Microbeads

Authors

  • A. I. Pravdyuk Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • Yu. A. Petrenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • V. S. Kholodnyy Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • V. P. Grischuk Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • A. Yu. Petrenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

alginate microbeads, mesenchymal stromal cells, encapsulation, cryopreservation

Abstract

Three-dimensional culture of mesenchymal stromal cells (MSC), encapsulated in alginate microbeads is a perspective direction of cell biology and tissue engineering, indicating the need of the development of their low temperature preservation methods. The study describes the possibility of cryopreservation of MSC within alginate microbeads by slow cooling under protection of DMSO. Comparison study of MSC within alginate microbeads and as non-encapsulated cell suspension during cryopreservation under protection of 10% DMSO did not show significant differences in cells’ cryosensitivity, while during cryopreservation with 5% DMSO encapsulated MSC had lower viability values. Survival and metabolic activity of MSC after cryopreservation within alginate microbeads depended on DMSO concentration in cryoprotective medium and rised with increase of DMSO concentration from 5% to 10%. Initiation of crystallization during slow program cooling under protection of 10% DMSO allowed to achieve maximal values of survival and metabolic activity of encapsulated cells. The results of this study could be used for the banking of MSC encapsulated into alginate microbeads.

Downloads

Published

2010-09-20

How to Cite

Pravdyuk, A. I., Petrenko, Y. A., Kholodnyy, V. S., Grischuk, V. P., & Petrenko, A. Y. (2010). Cryosensitivity of Mesenchymal Stromal Cells Encapsulated in Alginate Microbeads. Problems of Cryobiology and Cryomedicine, 20(3), 235–245. Retrieved from https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/184

Issue

Vol. 20 No. 3 (2010): Problems of Cryobiology

Section

Theoretical and Experimental Cryobiology

License

Copyright (c) 2020 A. I. Pravdyuk, Yu. A. Petrenko, V. S. Kholodnyy, V. P. Grischuk, A. Yu. Petrenko

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Authors who publish with this journal agree to the following terms:

  • Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  • Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  • Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).