Change of Antioxidative Properties of Human Placental Extracts after Freezing

Authors

  • S. L. Rozanova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • E. I. Naumenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • E. D. Rozanova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • O. A. Nardid Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

freeze-thawing, human placenta extract, antioxidant activity

Abstract

The experimental data, obtained under comparative evaluation of different freeze-thawing protocols' influence on human placenta extract antioxidant activity, estimated by capacity to reduce the ferric iron and ABTS+ radical, to chelate ferrous ions, to scavenge hydrogen peroxide and also by phenolic compound content, have been presented. Slow freezing of the extracts down to –20°С has been shown to cause the lowering of its activity, while rapid freezing down to –196°С increases the activity due to protein antioxidants' conformational changes.

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Published

2010-09-20

How to Cite

Rozanova, S. L., Naumenko, E. I., Rozanova, E. D., & Nardid, O. A. (2010). Change of Antioxidative Properties of Human Placental Extracts after Freezing. Problems of Cryobiology and Cryomedicine, 20(3), 288–296. Retrieved from https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/188

Issue

Vol. 20 No. 3 (2010): Problems of Cryobiology

Section

Theoretical and Experimental Cryobiology

License

Copyright (c) 2020 S. L. Rozanova, E. I. Naumenko, E. D. Rozanova, O. A. Nardid

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