Interactions of Cryoprotectants with Liposomes from Total Lipids of the Fowl Spermatozoa

Authors

  • T. P. Linnik Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • T. S. Dyubko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • I. N. Martynyuk Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • A. V. Tereschenko Institute of Domestic Bird Husbandry of Ukrainian Academy of Agricultural Sciences, Village Borki, Kharkov Region

Keywords:

fowl spermatozoa, lipids, liposomes, cryoprotectants, fluorescent probe

Abstract

Using the fluorescent probe FME we studied interactions of the cryoprotectants: ethylene glycol (EG), 1,2-propane diol (1,2-PD), N,N-dimethyl formamide (DMFA), N,N-dimethyl acetamide (DMAc), glycerol and dimethyl sulfoxide (DMSO) with liposomes made from the fowl spermatozoa total lipid (FSTL). It was established that the level of CP impacts on bilayer is determined by hydrophilic-hydrophobic balance of their molecules and showed a concentration dependence. The cryoprotectants are ranked by their increasing influence on FME fluorescence spectral parameters in RSTL liposomes as follows: glycerol ≤ EG < 1,2-PD < DMSO < DMAc ≤ DMFA, that corresponds the growth in their distribution coefficients in the system “water – non-polar phaseâ€. A mechanism of the cryoprotectant impacts on bonds forming bilayer is discussed.

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Published

2010-04-19

How to Cite

Linnik, T. P., Dyubko, T. S., Martynyuk, I. N., & Tereschenko, A. V. (2010). Interactions of Cryoprotectants with Liposomes from Total Lipids of the Fowl Spermatozoa. Problems of Cryobiology and Cryomedicine, 20(1), 34–46. Retrieved from https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/215

Issue

Vol. 20 No. 1 (2010): Problems of Cryobiology

Section

Theoretical and Experimental Cryobiology

License

Copyright (c) 2020 T. P. Linnik, T. S. Dyubko, I. N. Martynyuk, A. V. Tereschenko

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